Full metadata record
DC Field | Value | Language |
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dc.contributor.author | 허규정 | - |
dc.date.accessioned | 2017-01-18T02:01:28Z | - |
dc.date.available | 2017-01-18T02:01:28Z | - |
dc.date.issued | 2007 | - |
dc.identifier.issn | 1350-9047 | - |
dc.identifier.other | OAK-4434 | - |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/233945 | - |
dc.description.abstract | Cellular ionic homeostasis, fundamentally K+ homeostasis, has been implicated as a critical regulator of apoptosis. The intracellular K+ efflux on apoptotic insult and suppression of apoptosis by high concentration of extracellular K+ or after inhibition of this efflux by K+ channel blockers have established the crucial role of K+ in turning on the apoptotic machinery. Several contrasting observations have reported the antiapoptotic effect of intracellular K+ concentration to be the result of inhibition of cytochrome c release from mitochondria, but the exact inhibitory mechanism remains obscure. However, here we show the blockage of K+ efflux during apoptosis did not affect cytochrome c release from the mitochondria, still completely inhibited the formation of the apoptosome comprising Apaf-1, cytochrome c, caspase-9 and other accessories. As a consequence of this event, procaspase-9, -3, -8 and other death-related proteins were not processed. Furthermore, physiological concentrations of K+ also inhibited the processing of procaspase-3 by purified caspase-8 or -9, the nucleosomal DNA fragmentation by purified DFF40/CAD and the nuclear fragmentation to varying extents. Altogether, these findings suggest that the efflux of K+ is prerequisite not only for the formation of the apoptosome but also for the downstream apoptotic signal-transduction pathways. | - |
dc.language | English | - |
dc.title | Intracellular K+ inhibits apoptosis by suppressing the Apaf-1 apoptosome formation and subsequent downstream pathways but not cytochrome c release | - |
dc.type | Article | - |
dc.relation.issue | 12 | - |
dc.relation.volume | 14 | - |
dc.relation.index | SCIE | - |
dc.relation.index | SCOPUS | - |
dc.relation.startpage | 2068 | - |
dc.relation.lastpage | 2075 | - |
dc.relation.journaltitle | Cell Death and Differentiation | - |
dc.identifier.doi | 10.1038/sj.cdd.4402221 | - |
dc.identifier.wosid | WOS:000251213200012 | - |
dc.identifier.scopusid | 2-s2.0-36248979229 | - |
dc.author.google | Karki P. | - |
dc.author.google | Seong C. | - |
dc.author.google | Kim J.-E. | - |
dc.author.google | Hur K. | - |
dc.author.google | Shin S.Y. | - |
dc.author.google | Lee J.S. | - |
dc.author.google | Cho B. | - |
dc.author.google | Park I.-S. | - |
dc.contributor.scopusid | 허규정(7005230301) | - |
dc.date.modifydate | 20190301081000 | - |