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BMI-1 interacts with sMEK1 and inactivates sMEK1-induced apoptotic cell death

Title
BMI-1 interacts with sMEK1 and inactivates sMEK1-induced apoptotic cell death
Authors
Kim, Boh-RamKwon, YoungjooRho, Seung Bae
Ewha Authors
권영주
SCOPUS Author ID
권영주scopus
Issue Date
2017
Journal Title
ONCOLOGY REPORTS
ISSN
1021-335XJCR Link1791-2431JCR Link
Citation
vol. 37, no. 1, pp. 579 - 586
Keywords
BMI-1sMEK1protein-protein interactionpro-apoptotic activityovarian tumor metastasis
Publisher
SPANDIDOS PUBL LTD
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
The B lymphoma Mo-MLV insertion region 1 homolog (BMI-1) protein is activated in various types of tumors and associated with cancer development and tumor progression. However, the working role of BMI-1 in cellular signaling is not understood completely. In this study, we revealed one possible biologic mechanism of BMI-1 in cancer progression in vitro using a human ovarian tumor cell system. Suppressor of MEK1 (sMEK1), a pivotal regulator involved in the cellular biological response mechanism, was identified as a BMI-1-binding protein. Ectopic expression of BMI-1 activated cell growth by reducing sMEK1-stimulated apoptotic cell death and suppressing p21, p27 and p53 expression, while enhancing cyclin Dl, CDK4 and Bcl-2 expression. The effect of BMI-1 on cell cycle and apoptotic regulatory proteins was also confirmed via silencing of BMI-1 expression. Subsequently, the promoter activities of p21 and p53 were inactivated significantly. However, BMI-1 overexpression noticeably increased Bcl-2 and NF-kappa B activities. In addition, BMI-1 activated the PI3K/mTOR/4E-BP1 signaling pathways, and sMEK1 significantly inhibited BMI-1-stimulated oncogenesis. These insights provide evidence that BMI-1 activates cell growth and suppresses apoptosis. Collectively, our data indicate that BMI-1 plays a pivotal role in the progression of ovarian cancer, thus representing a novel target for antitumor therapy of ovarian cancer.
DOI
10.3892/or.2016.5262
Appears in Collections:
약학대학 > 약학과 > Journal papers
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