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BMI-1 interacts with sMEK1 and inactivates sMEK1-induced apoptotic cell death
- Title
- BMI-1 interacts with sMEK1 and inactivates sMEK1-induced apoptotic cell death
- Authors
- Kim, Boh-Ram; Kwon, Youngjoo; Rho, Seung Bae
- Ewha Authors
- 권영주
- SCOPUS Author ID
- 권영주
- Issue Date
- 2017
- Journal Title
- ONCOLOGY REPORTS
- ISSN
- 1021-335X
1791-2431
- Citation
- ONCOLOGY REPORTS vol. 37, no. 1, pp. 579 - 586
- Keywords
- BMI-1; sMEK1; protein-protein interaction; pro-apoptotic activity; ovarian tumor metastasis
- Publisher
- SPANDIDOS PUBL LTD
- Indexed
- SCIE; SCOPUS
- Document Type
- Article
- Abstract
- The B lymphoma Mo-MLV insertion region 1 homolog (BMI-1) protein is activated in various types of tumors and associated with cancer development and tumor progression. However, the working role of BMI-1 in cellular signaling is not understood completely. In this study, we revealed one possible biologic mechanism of BMI-1 in cancer progression in vitro using a human ovarian tumor cell system. Suppressor of MEK1 (sMEK1), a pivotal regulator involved in the cellular biological response mechanism, was identified as a BMI-1-binding protein. Ectopic expression of BMI-1 activated cell growth by reducing sMEK1-stimulated apoptotic cell death and suppressing p21, p27 and p53 expression, while enhancing cyclin Dl, CDK4 and Bcl-2 expression. The effect of BMI-1 on cell cycle and apoptotic regulatory proteins was also confirmed via silencing of BMI-1 expression. Subsequently, the promoter activities of p21 and p53 were inactivated significantly. However, BMI-1 overexpression noticeably increased Bcl-2 and NF-kappa B activities. In addition, BMI-1 activated the PI3K/mTOR/4E-BP1 signaling pathways, and sMEK1 significantly inhibited BMI-1-stimulated oncogenesis. These insights provide evidence that BMI-1 activates cell growth and suppresses apoptosis. Collectively, our data indicate that BMI-1 plays a pivotal role in the progression of ovarian cancer, thus representing a novel target for antitumor therapy of ovarian cancer.
- DOI
- 10.3892/or.2016.5262
- Appears in Collections:
- 약학대학 > 약학과 > Journal papers
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