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Silencing the livin gene enhances the cytotoxic effects of anticancer drugs on colon cancer cells

Title
Silencing the livin gene enhances the cytotoxic effects of anticancer drugs on colon cancer cells
Authors
Oh B.Y.Kim K.H.Chung S.S.Lee R.-A.
Ewha Authors
김광호이령아정순섭
SCOPUS Author ID
김광호scopus; 이령아scopus; 정순섭scopus
Issue Date
2016
Journal Title
Annals of Surgical Treatment and Research
ISSN
2288-6575JCR Link
Citation
vol. 91, no. 6, pp. 273 - 277
Keywords
ApoptosisColorectal neoplasmsLivin proteinOxaliplatin
Publisher
Korean Surgical Society
Indexed
SCIE; SCOPUS; KCI WOS scopus
Abstract
Purpose: Livin is associated with drug response in several cancers. The aim of this study was to investigate the effect of silencing the livin gene expression on anticancer drug response in colorectal cancer. Methods: siRNA was transfected at different concentrations (0, 10, and 30nM) into HCT116 cells, then cells were treated with either 5-fluorouracil (FU)/leucovorin (LV) or oxaliplatin (L-OHP)/5-FU/LV. Cellular viability and apoptosis were evaluated following silencing of livin gene expression combined with treatment with anticancer drugs. Results: Livin gene expression was effectively suppressed by 30nM siRNA compared with control and 10nM siRNA. The 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide assay showed that proliferation was effectively inhibited in cells treated with a combination of both siRNA and an anticancer drug, compared to cells treated with siRNA-Livin or anticancer drug alone. In particular, the combination of 30nM siRNA and L-OHP/5-FU/LV resulted in a 93.8% and 91.4% decrease, compared to untreated control or L-OHP/5-FU/LV alone, respectively. Cellular proliferation was most effectively suppressed by a combination of 30nM of siRNA and L-OHP/5-FU/LV compared to other combinations. Conclusion: siRNA-mediated down-regulation of livin gene expression could significantly suppress colon cancer growth and enhance the cytotoxic effects of anticancer drugs such as 5-FU and L-OHP. The results of this study suggest that silencing livin gene expression in combination with treatment with anticancer drugs might be a novel cancer therapy for colorectal cancer.
DOI
10.4174/astr.2016.91.6.273
Appears in Collections:
의과대학 > 의학과 > Journal papers
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