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Activating signal cointegrator 2 belongs to a novel steady-state complex that contains a subset of trithorax group proteins

Title
Activating signal cointegrator 2 belongs to a novel steady-state complex that contains a subset of trithorax group proteins
Authors
Goo Y.-H.Chang Sohn Y.Kim D.-H.Kim S.-W.Kang M.-J.Jung D.-J.Kwak E.Barlev N.A.Berger S.L.Chow V.T.Roeder R.G.Azorsa D.O.Meltzer P.S.Suh P.-G.Song E.J.Lee K.-J.Lee Y.C.Lee J.W.
Ewha Authors
이공주
SCOPUS Author ID
이공주scopus
Issue Date
2003
Journal Title
Molecular and Cellular Biology
ISSN
0270-7306JCR Link
Citation
vol. 23, no. 1, pp. 140 - 149
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
Many transcription coactivators interact with nuclear receptors in a ligand- and C-terminal transactivation function (AF2)-dependent manner. These include activating signal cointegrator 2 (ASC-2), a recently isolated transcriptional coactivator molecule, which is amplified in human cancers and stimulates transactivation by nuclear receptors and numerous other transcription factors. In this report, we show that ASC-2 belongs to a steady-state complex of approximately 2 MDa (ASC-2 complex [ASCOM]) in HeLa nuclei. ASCOM contains retinoblastoma-binding protein RBQ-3, α/β-tubulins, and trithorax group proteins ALR-1, ALR-2, HALR, and ASH2. In particular, ALR-1/2 and HALR contain a highly conserved 130- to 140-amino-acid motif termed the SET domain, which was recently implicated in histone H3 lysine-specific methylation activities. Indeed, recombinant ALR-1, HALR, and immunopurified ASCOM exhibit very weak but specific H3-lysine 4 methylation activities in vitro, and transactivation by retinoic acid receptor appears to involve ligand-dependent recruitment of ASCOM and subsequent transient H3-lysine 4 methylation of the promoter region in vivo. Thus, ASCOM may represent a distinct coactivator complex of nuclear receptors. Further characterization of ASCOM will lead to a better understanding of how nuclear receptors and other transcription factors mediate transcriptional activation.
DOI
10.1128/MCB.23.1.140-149.2003
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