Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 서석효 | - |
dc.date.accessioned | 2017-01-05T02:01:12Z | - |
dc.date.available | 2017-01-05T02:01:12Z | - |
dc.date.issued | 2004 | - |
dc.identifier.issn | 0031-6768 | - |
dc.identifier.other | OAK-1780 | - |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/233670 | - |
dc.description.abstract | We employed the patch-clamp technique to investigate a Ca 2+-activated K+ (KCa) current in cultured mouse aortic endothelial cells (MAECs). In the whole-cell mode, an increase in cytosolic [Ca2+] ([Ca2+]i) to 2 μM activated an outwards current. The [K+]o-dependent change of the reversal potentials agreed well with the predicted Nernstian relation, suggesting that it was a KCa current. The Hill coefficient (4) and EC50 (740 nM) were obtained from the current/[Ca2+] i relationship. Iberiotoxin (50 nM) or apamin (200 nM) failed to inhibit the current, whereas TEA (10 mM) suppressed the current to 73.6 ±1.6% of control (n=9). The intermediate-conductance, Ca 2+-activated K+ (IKCa) channel blockers charybdotoxin (50 nM), clotrimazole (10 μM) and econazole (10 μM) inhibited the KCa current to 10.5±1.3% (n=6), 16.6±3. 1% (n=6), and 19.3±2.5% (n=5) of control, respectively. The EK Ca channel openers chlorzoxazone, zoxazolamine and 1-ethyl-2-benz-imidazolinone and the Ca2+-activated Cl- channel blocker niflumic acid activated the KCa current. In inside-out patches, the single-channel conductance was 17.7 pS in symmetrical K+ solutions. RT-PCR analysis showed transcripts of the murine IK1 channel (mIK1) in MAECs. The IKCa channel blockers inhibited the ATP-induced [Ca2+]i increase in MAECs and the endothelium-dependent relaxation of mouse aortic rings. In addition, the IK Ca channel openers augmented ATP-induced [Ca2+] i increase in MAECs and evoked endothelium-dependent relaxation of mouse aorta. These results suggest that an mIK1-like channel mediates the native IKCa current in MAECs and may contribute to endothelium-dependent relaxation by modulating MAEC [Ca2+] i. | - |
dc.language | English | - |
dc.title | Characteristics and a functional implication of Ca2+-activated K+ current in mouse aortic endothelial cells | - |
dc.type | Article | - |
dc.relation.issue | 4 | - |
dc.relation.volume | 447 | - |
dc.relation.index | SCI | - |
dc.relation.index | SCIE | - |
dc.relation.index | SCOPUS | - |
dc.relation.startpage | 426 | - |
dc.relation.lastpage | 435 | - |
dc.relation.journaltitle | Pflugers Archiv European Journal of Physiology | - |
dc.identifier.doi | 10.1007/s00424-003-1201-1 | - |
dc.identifier.wosid | WOS:000187548800008 | - |
dc.identifier.scopusid | 2-s2.0-0942279568 | - |
dc.author.google | Ahn S.C. | - |
dc.author.google | Seol G.H. | - |
dc.author.google | Kim J.A. | - |
dc.author.google | Suh S.H. | - |
dc.contributor.scopusid | 서석효(55666113100) | - |
dc.date.modifydate | 20230901081001 | - |