Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 이공주 | * |
dc.contributor.author | 이수영 | * |
dc.date.accessioned | 2017-01-05T02:01:11Z | - |
dc.date.available | 2017-01-05T02:01:11Z | - |
dc.date.issued | 2004 | * |
dc.identifier.issn | 0021-9258 | * |
dc.identifier.other | OAK-1808 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/233661 | - |
dc.description.abstract | Fas-associated factor-1 (FAF1) is a Fas-binding proapoptotic protein that is a component of the death-inducing signaling complex in Fas-mediated apoptosis. Here, we show that FAF1 is involved in negative regulation of NF-κB activation. Overexpression of FAF1 decreased the basal level of NF-κB activity in 293 cells. NF-κB activation induced by tumor necrosis factor (TNF)-α, interleukin-1β, and lipopolysaccharide was also inhibited by FAF1 overexpression. Moreover, FAF1 suppressed NF-κB activation induced by transducers of diverse NF-κB-activating signals such as TNF receptor-associated factor-2 and -6, MEKK1, and IκB kinase-β as well as NF-κB p65, one of the end point molecules in the NF-κB activation pathway, suggesting that NF-κB p65 might be a target molecule upon which FAF1 acts. Subsequent study disclosed that FAF1 physically interacts with NF-κB p65 and that the binding domain of FAF1 is the death effector domain (DED)-interacting domain (amino acids 181-381), where DEDs of the Fas-associated death domain protein and caspase-8 interact. The NF-κB activity-modulating potential of FAF1 was also mapped to the DED-interacting domain. Finally, overexpression of FAF1 prevented translocation of NF-κB p65 into the nucleus and decreased its DNA-binding activity upon TNFα treatment. This study presents a novel function of FAF1, in addition to the previously known function as a component of the Fas death-inducing signaling complex, i.e. NF-κB activity suppressor by cytoplasmic retention of NF-κB p65 via physical interaction. | * |
dc.language | English | * |
dc.title | Fas-associated Factor-1 Inhibits Nuclear Factor-κB (NF-κB) Activity by Interfering with Nuclear Translocation of the RelA (p65) Subunit of NF-κB | * |
dc.type | Article | * |
dc.relation.issue | 4 | * |
dc.relation.volume | 279 | * |
dc.relation.index | SCI | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.startpage | 2544 | * |
dc.relation.lastpage | 2549 | * |
dc.relation.journaltitle | Journal of Biological Chemistry | * |
dc.identifier.doi | 10.1074/jbc.M304565200 | * |
dc.identifier.wosid | WOS:000188211300027 | * |
dc.identifier.scopusid | 2-s2.0-1642576087 | * |
dc.author.google | Park M.-Y. | * |
dc.author.google | Jang H.D. | * |
dc.author.google | Lee S.Y. | * |
dc.author.google | Lee K.-J. | * |
dc.author.google | Kim E. | * |
dc.contributor.scopusid | 이공주(7501497635;57191532162) | * |
dc.contributor.scopusid | 이수영(53980218900;7409697278) | * |
dc.date.modifydate | 20240415140424 | * |