Full metadata record
DC Field | Value | Language |
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dc.contributor.author | 장준 | * |
dc.date.accessioned | 2017-01-05T02:01:03Z | - |
dc.date.available | 2017-01-05T02:01:03Z | - |
dc.date.issued | 2008 | * |
dc.identifier.issn | 0022-538X | * |
dc.identifier.other | OAK-4634 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/233586 | - |
dc.description.abstract | Respiratory syncytial virus (RSV) is a major cause of severe lower respiratory tract disease in infancy and early childhood. Despite its importance as a pathogen, there is no licensed vaccine against RSV. The G glycoprotein of RSV, a major attachment protein, is a potentially important target for protective antiviral immune responses. Here, a recombinant replication-deficient adenovirus-based vaccine, rAd/3xG, expressing the soluble core domain of G glycoprotein (amino acids 130 to 230) engineered by codon optimization and tandem repetition for higher-level expression, was constructed and evaluated for its potential as an RSV vaccine in a murine model. A single intranasal immunization with rAd/3xG provided potent protection against RSV challenge which lasted for more than 10 weeks. Strong mucosal immunoglobulin A responses were also induced by a single intranasal immunization but not by intramuscular or oral administration of rAd/3xG. Interestingly, neither gamma interferon- nor interleukin-4-producing CD4 T cells directed to I-Ed-restricted epitope were detected in the lungs of rAd/3xG-immune mice upon challenge, whereas priming with vaccinia virus expressing RSV G (vvG) elicited strong Th1/Th2 mixed CD4 T-cell responses. Lung eosinophilia and vaccine-induced weight loss were significantly lower in the rAd/3xG-immune group than in the wG-primed group. Together, our data demonstrate that a single intranasal administration of rAd/3xG elicits beneficial protective immunity and represents a promising vaccine regimen against RSV infection. Copyright © 2008, American Society for Microbiology. All Rights Reserved. | * |
dc.language | English | * |
dc.title | Single intranasal immunization with recombinant adenovirus-based vaccine induces protective immunity against respiratory syncytial virus infection | * |
dc.type | Article | * |
dc.relation.issue | 5 | * |
dc.relation.volume | 82 | * |
dc.relation.index | SCI | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.startpage | 2350 | * |
dc.relation.lastpage | 2357 | * |
dc.relation.journaltitle | Journal of Virology | * |
dc.identifier.doi | 10.1128/JVI.02372-07 | * |
dc.identifier.wosid | WOS:000253481000030 | * |
dc.identifier.scopusid | 2-s2.0-39749187575 | * |
dc.author.google | Yu J.-R. | * |
dc.author.google | Kim S. | * |
dc.author.google | Lee J.-B. | * |
dc.author.google | Chang J. | * |
dc.contributor.scopusid | 장준(8735999100) | * |
dc.date.modifydate | 20231120165756 | * |