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Distinct locomotive patterns of granulocytes, monocytes and lymphocytes in a stable concentration gradient of chemokines
- Distinct locomotive patterns of granulocytes, monocytes and lymphocytes in a stable concentration gradient of chemokines
- Bae S.Y.; Jung Y.J.; Woo S.Y.; Park M.H.; Seoh J.Y.; Ryu K.H.
- Ewha Authors
- 서주영; 유경하; 박미혜; 우소연
- SCOPUS Author ID
- 서주영; 유경하; 박미혜; 우소연
- Issue Date
- Journal Title
- International Journal of Laboratory Hematology
- vol. 30, no. 2, pp. 139 - 148
- SCI; SCIE; SCOPUS
- The pattern of leukocyte locomotion can be changed in many pathological situations, but its accurate analysis is difficult because of technological limitation. In the present study, by using a newly developed time-lapse videomicroscopic technique, we have analyzed the locomotive patterns of leukocytes in a stable concentration gradient of chemokines. Granulocytes, monocytes, and lymphocytes were purified from adult human peripheral blood. Locomotive behavior of the leukocytes was analyzed by an optical assay using a microchannel producing a stable concentration gradient of chemokines. Videomicroscopic analysis showed distinct locomotive patterns of granulocytes, monocytes, and lymphocytes. Granulocytes were intrinsically motile, vigorously moving in random direction without any chemokine stimulation. Upon stimulation with CXCL8/IL-8, the speed of migration was increased from 13.3 ± 2.8 to 19.4 ± 2.5 μm/min (P = 0.002, n = 100) and they moved toward the chemokine, although many cells still wandered very much. Stimulation with CCL5/RANTES or CXCL12/SDF-1α induced similar changes in locomotive pattern. On the other hand, most lymphocytes did not polarize or move spontaneously without chemokine stimulation. Stimulation with CXCL12 induced directional migration in 37% of the lymphocytes at a speed of 9.6 ± 1.6 μm/min (n = 100). The movement pattern of monocytes was similar to that of granulocytes in that they tend to become polarized and move spontaneously, but they moved at a very slow speed ranging from 3.9 to 4.2 μm/min even with chemokine stimulation. The new optical assay may be useful for many diagnostic as well as basic studies. © 2007 The Authors.
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