Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 오구택 | * |
dc.date.accessioned | 2017-01-05T02:01:53Z | - |
dc.date.available | 2017-01-05T02:01:53Z | - |
dc.date.issued | 2008 | * |
dc.identifier.issn | 0013-7227 | * |
dc.identifier.other | OAK-4824 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/233494 | - |
dc.description.abstract | To study the role of the epithelial calcium channel transient receptor potential vanilloid type 6 (TRPV6) and the calcium-binding protein calbindin-D9k in intestinal calcium absorption, TRPV6 knockout (KO), calbindin-D9k KO, and TRPV6/calbindin-D9k double-KO (DKO) mice were generated. TRPV6 KO, calbindin-D9k KO, and TRPV6/calbindin-D9k DKO mice have serum calcium levels similar to those of wild-type (WT) mice (∼10 mg Ca2+/dl). In the TRPV6 KO and the DKO mice, however, there is a 1.8-fold increase in serum PTH levels (P < 0.05 compared with WT). Active intestinal calcium transport was measured using the everted gut sac method. Under low dietary calcium conditions there was a 4.1-, 2.9-, and 3.9-fold increase in calcium transport in the duodenum of WT, TRPV6 KO, and calbindin-D9k KO mice, respectively (n = 8-22 per group; P > 0.1, WT vs. calbindin-D9k KO, and P < 0.05, WT vs. TRPV6 KO on the low-calcium diet). Duodenal calcium transport was increased 2.1-fold in the TRPV6/calbindin-D9k DKO mice fed the low-calcium diet (P < 0.05, WT vs. DKO). Active calcium transport was not stimulated by low dietary calcium in the ileum of the WT or KO mice. 1,25-Dihydroxyvitamin D 3 administration to vitamin D-deficient null mutant and WT mice also resulted in a significant increase in duodenal calcium transport (1.4- to 2.0-fold, P < 0.05 compared with vitamin D-deficient mice). This study provides evidence for the first time using null mutant mice that significant active intestinal calcium transport occurs in the absence of TRPV6 and calbindin-D9k, thus challenging the dogma that TRPV6 and calbindin-D9k are essential for vitamin D-induced active intestinal calcium transport. Copyright © 2008 by The Endocrine Society. | * |
dc.language | English | * |
dc.title | Active intestinal calcium transport in the absence of transient receptor potential vanilloid type 6 and calbindin-D9k | * |
dc.type | Article | * |
dc.relation.issue | 6 | * |
dc.relation.volume | 149 | * |
dc.relation.index | SCI | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.startpage | 3196 | * |
dc.relation.lastpage | 3205 | * |
dc.relation.journaltitle | Endocrinology | * |
dc.identifier.doi | 10.1210/en.2007-1655 | * |
dc.identifier.wosid | WOS:000256053100059 | * |
dc.identifier.scopusid | 2-s2.0-44249113292 | * |
dc.author.google | Benn B.S. | * |
dc.author.google | Ajibade D. | * |
dc.author.google | Porta A. | * |
dc.author.google | Dhawan P. | * |
dc.author.google | Hediger M. | * |
dc.author.google | Peng J.-B. | * |
dc.author.google | Jiang Y. | * |
dc.author.google | Goo T.O. | * |
dc.author.google | Jeung E.-B. | * |
dc.author.google | Lieben L. | * |
dc.author.google | Bouillon R. | * |
dc.author.google | Carmeliet G. | * |
dc.author.google | Christakos S. | * |
dc.contributor.scopusid | 오구택(7007056663) | * |
dc.date.modifydate | 20240123094756 | * |