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Active intestinal calcium transport in the absence of transient receptor potential vanilloid type 6 and calbindin-D9k

Title
Active intestinal calcium transport in the absence of transient receptor potential vanilloid type 6 and calbindin-D9k
Authors
Benn B.S.Ajibade D.Porta A.Dhawan P.Hediger M.Peng J.-B.Jiang Y.Goo T.O.Jeung E.-B.Lieben L.Bouillon R.Carmeliet G.Christakos S.
Ewha Authors
오구택
SCOPUS Author ID
오구택scopus
Issue Date
2008
Journal Title
Endocrinology
ISSN
0013-7227JCR Link
Citation
vol. 149, no. 6, pp. 3196 - 3205
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
To study the role of the epithelial calcium channel transient receptor potential vanilloid type 6 (TRPV6) and the calcium-binding protein calbindin-D9k in intestinal calcium absorption, TRPV6 knockout (KO), calbindin-D9k KO, and TRPV6/calbindin-D9k double-KO (DKO) mice were generated. TRPV6 KO, calbindin-D9k KO, and TRPV6/calbindin-D9k DKO mice have serum calcium levels similar to those of wild-type (WT) mice (∼10 mg Ca2+/dl). In the TRPV6 KO and the DKO mice, however, there is a 1.8-fold increase in serum PTH levels (P < 0.05 compared with WT). Active intestinal calcium transport was measured using the everted gut sac method. Under low dietary calcium conditions there was a 4.1-, 2.9-, and 3.9-fold increase in calcium transport in the duodenum of WT, TRPV6 KO, and calbindin-D9k KO mice, respectively (n = 8-22 per group; P > 0.1, WT vs. calbindin-D9k KO, and P < 0.05, WT vs. TRPV6 KO on the low-calcium diet). Duodenal calcium transport was increased 2.1-fold in the TRPV6/calbindin-D9k DKO mice fed the low-calcium diet (P < 0.05, WT vs. DKO). Active calcium transport was not stimulated by low dietary calcium in the ileum of the WT or KO mice. 1,25-Dihydroxyvitamin D 3 administration to vitamin D-deficient null mutant and WT mice also resulted in a significant increase in duodenal calcium transport (1.4- to 2.0-fold, P < 0.05 compared with vitamin D-deficient mice). This study provides evidence for the first time using null mutant mice that significant active intestinal calcium transport occurs in the absence of TRPV6 and calbindin-D9k, thus challenging the dogma that TRPV6 and calbindin-D9k are essential for vitamin D-induced active intestinal calcium transport. Copyright © 2008 by The Endocrine Society.
DOI
10.1210/en.2007-1655
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자연과학대학 > 생명과학전공 > Journal papers
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