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A Validated LC Method for Simultaneous Determination of Phenolic, Coumarin and Phthalide Compounds in the Ethanolic Extract of Angelica tenuissima

Title
A Validated LC Method for Simultaneous Determination of Phenolic, Coumarin and Phthalide Compounds in the Ethanolic Extract of Angelica tenuissima
Authors
Islam M.N.Nam J.-W.Lee J.Seo E.-K.Han S.B.Kim D.-H.Jin C.Yoo H.H.
Ewha Authors
서은경
SCOPUS Author ID
서은경scopus
Issue Date
2009
Journal Title
Chromatographia
ISSN
0009-5893JCR Link
Citation
pp. 1 - 7
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
In this study, an LC method for the simultaneous determination of six bioactive compounds from Angelica tenuissima, namely chlorogenic acid, ferulic acid, Z-ligustilide, nodakenin, decursin and decursinol angelate was developed and validated. Chromatographic analysis was carried out on a C18 column with a mobile phase consisting of 0.1% formic acid, methanol and acetonitrile at a flow rate of 0.8 mL min-1 and the effluent from the column was monitored by UV detector at 325 nm. The excellent linear behavior was observed over the investigated concentration range for reported compounds. The intra- and inter-day precision over the concentration range of compounds were lower than 1.7% (as relative standard deviation), and accuracy was between 97.2 and 106.0%. These results showed that the developed method is accurate, reproducible, and consequently applicable for the quantitation of bioactive components from the ethanolic extract of Angelica tenuissima Nakai. © 2009 Vieweg+Teubner

GWV Fachverlage GmbH.
DOI
10.1365/s10337-009-1263-0
Appears in Collections:
약학대학 > 약학과 > Journal papers
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