Full metadata record
DC Field | Value | Language |
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dc.contributor.author | 박혜영 | - |
dc.date.accessioned | 2016-12-28T02:12:40Z | - |
dc.date.available | 2016-12-28T02:12:40Z | - |
dc.date.issued | 2016 | - |
dc.identifier.issn | 0006-291X | - |
dc.identifier.issn | 1090-2104 | - |
dc.identifier.other | OAK-19680 | - |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/233364 | - |
dc.description.abstract | 5-Lipoxygenase (5-LOX) is an enzyme that converts arachidonic acid from the cell membrane into leukotriene, a signal lipid mediator. 5-LOX deficiency markedly attenuates the formation of aneurysms in knockout mice. In addition, Zileuton, a clinical drug targeting 5-LOX, is used for treatment of asthma. However, it is unclear whether 5-LOX inhibition results in anti-angiogenic effects for applications in cancer therapy. To explore the roles of 5-LOX in angiogenesis and its potential as a therapeutic target in cancer, the effects of a newly synthesized 5-LOX inhibitor, F3, on in vitro and in vivo angiogenesis were investigated. The results showed that 5-LOX inhibition by F3 suppressed in vitro vascular endothelial growth factor (VEGF)-induced tube formation and chemo-invasion of endothelial cells (ECs). 5-LOX inhibition also decreased VEGF-induced extracellular signal-regulated kinase (ERK) phosphorylation in ECs. Notably, 5-LOX knockdown phenocopied the anti-angiogenic activity of the 5-LOX inhibitor F3 in a concentration-dependent manner. F3 did not affect the activities of VEGF receptor 2 or AKT. In vivo, the compound significantly inhibited the formation of the chorioallantoic membrane at nontoxic doses. These results demonstrated that 5-LOX played an important role in angiogenesis and that its inhibitor F3 could be a new anti-angiogenic agent targeting VEGF signaling. | - |
dc.language | English | - |
dc.publisher | ACADEMIC PRESS INC ELSEVIER SCIENCE | - |
dc.subject | 5-Lipoxygenase | - |
dc.subject | Anti-angiogenic effects | - |
dc.subject | Vascular endothelial growth factor signaling | - |
dc.title | Inhibition of 5-lipoxygenase suppresses vascular endothelial growth factor-induced angiogenesis in endothelial cells | - |
dc.type | Article | - |
dc.relation.issue | 3 | - |
dc.relation.volume | 478 | - |
dc.relation.index | SCIE | - |
dc.relation.index | SCOPUS | - |
dc.relation.startpage | 1117 | - |
dc.relation.lastpage | 1122 | - |
dc.relation.journaltitle | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | - |
dc.identifier.doi | 10.1016/j.bbrc.2016.08.078 | - |
dc.identifier.wosid | WOS:000397715400016 | - |
dc.identifier.scopusid | 2-s2.0-84993958529 | - |
dc.author.google | Kim, Tae Young | - |
dc.author.google | Kim, Joohye | - |
dc.author.google | Choo, Hea-Young Park | - |
dc.author.google | Kwon, Ho Jeong | - |
dc.contributor.scopusid | 박혜영(34972649500;57200273796) | - |
dc.date.modifydate | 20230411110509 | - |