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Tonsil-derived mesenchymal stem cells differentiate into a schwann cell phenotype and promote peripheral nerve regeneration

Title
Tonsil-derived mesenchymal stem cells differentiate into a schwann cell phenotype and promote peripheral nerve regeneration
Authors
Jung N.Park S.Choi Y.Park J.-W.Hong Y.B.Park H.H.C.Yu Y.Kwak G.Kim H.S.Ryu K.-H.Kim J.K.Jo I.Choi B.-O.Jung S.-C.
Ewha Authors
유경하김한수정성철김재광조인호박주원유연실박세영
SCOPUS Author ID
유경하scopus; 김한수scopus; 정성철scopus; 김재광scopus; 조인호scopus; 박주원scopus; 유연실scopus; 박세영scopus
Issue Date
2016
Journal Title
International Journal of Molecular Sciences
ISSN
1661-6596JCR Link
Citation
vol. 17, no. 11
Keywords
DifferentiationPeripheral nerveRegenerationSchwann cellTonsil-derived mesenchymal stem cells
Publisher
MDPI AG
Indexed
SCIE; SCOPUS WOS scopus
Abstract
Schwann cells (SCs), which produce neurotropic factors and adhesive molecules, have been reported previously to contribute to structural support and guidance during axonal regeneration; therefore, they are potentially a crucial target in the restoration of injured nervous tissues. Autologous SC transplantation has been performed and has shown promising clinical results for treating nerve injuries and donor site morbidity, and insufficient production of the cells have been considered as a major issue. Here, we performed differentiation of tonsil-derived mesenchymal stem cells (T-MSCs) into SC-like cells (T-MSC-SCs), to evaluate T-MSC-SCs as an alternative to SCs. Using SC markers such as CAD19, GFAP, MBP, NGFR, S100B, and KROX20 during quantitative real-time PCR we detected the upregulation of NGFR, S100B, and KROX20 and the downregulation of CAD19 and MBP at the fully differentiated stage. Furthermore, we found myelination of axons when differentiated SCs were cocultured with mouse dorsal root ganglion neurons. The application of T-MSC-SCs to a mouse model of sciatic nerve injury produced marked improvements in gait and promoted regeneration of damaged nerves. Thus, the transplantation of human T-MSCs might be suitable for assisting in peripheral nerve regeneration. © 2016 by the authors; licensee MDPI, Basel, Switzerland.
DOI
10.3390/ijms17111867
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의학전문대학원 > 의학과 > Journal papers
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