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Metabolic Profiling of Liver Tissue in Diabetic Mice Treated with Artemisia Capillaris and Alisma Rhizome Using LC-MS and CE-MS

Title
Metabolic Profiling of Liver Tissue in Diabetic Mice Treated with Artemisia Capillaris and Alisma Rhizome Using LC-MS and CE-MS
Authors
Kim, YumiLee, In-SeungKim, Kang-HoonPark, JiyoungLee, Ji-HyunBang, EunjungJang, Hyeung-JinNa, Yun-Cheol
Ewha Authors
나윤철
Issue Date
2016
Journal Title
AMERICAN JOURNAL OF CHINESE MEDICINE
ISSN
0192-415XJCR Link1793-6853JCR Link
Citation
vol. 44, no. 8, pp. 1639 - 1661
Keywords
Hepatic MetaboliteNatural ProductArtemisia CapillarisAlisma RhizomePhytotherapyType 2 Diabetes
Publisher
WORLD SCIENTIFIC PUBL CO PTE LTD
Indexed
SCIE; SCOPUS WOS scopus
Abstract
Artemisia Capillaris (AC) and Alisma Rhizome (AR) are natural products for the treatment of liver disorders in oriental medicine clinics. Here, we report metabolomic changes in the evaluation of the treatment effects of AC and AR on fatty livers in diabetic mice, along with a proposition of the underlying metabolic pathway. Hydrophobic and hydrophilic metabolites extracted from mouse livers were analyzed using HPLC-QTOF and CE-QTOF, respectively, to generate metabolic profiles. Statistical analysis of the metabolites by PLS-DA and OPLA-DA fairly discriminated between the diabetic, and the AC- and AR-treated mice groups. Various PEs mostly contributed to the discrimination of the diabetic mice from the normal mice, and besides, DG (18: 1/16: 0), TG (16: 1/16: 1/20: 1), PE (21: 0/20: 5), and PA (18: 0/21: 0) were also associated with discrimination by s-plot. Nevertheless, the effects of AC and AR treatment were indistinct with respect to lipid metabolites. Of the 97 polar metabolites extracted from the CE-MS data, 40 compounds related to amino acid, central carbon, lipid, purine, and pyrimidine metabolism, with p values less than 0.05, were shown to contribute to liver dysregulation. Following treatment with AC and AR, the metabolites belonging to purine metabolism preferentially recovered to the metabolic state of the normal mice. The AMP/ATP ratio of cellular energy homeostasis in AR-treated mice was more apparently increased (p < 0: 05) than that of AC-treated mice. On the other hand, amino acids, which showed the main alterations in diabetic mice, did not return to the normal levels upon treatment with AR or AC. In terms of metabolomics, AR was a more effective natural product in the treatment of liver dysfunction than AC. These results may provide putative biomarkers for the prognosis of fatty liver disorder following treatment with AC and AR extracts.
DOI
10.1142/S0192415X16500920
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자연과학대학 > 화학·나노과학전공 > Journal papers
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