Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 이동현 | * |
dc.contributor.author | 윤하나 | * |
dc.contributor.author | 박상희 | * |
dc.contributor.author | 안영호 | * |
dc.contributor.author | 김광현 | * |
dc.date.accessioned | 2016-12-27T02:12:26Z | - |
dc.date.available | 2016-12-27T02:12:26Z | - |
dc.date.issued | 2016 | * |
dc.identifier.issn | 1949-2553 | * |
dc.identifier.other | OAK-19695 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/233164 | - |
dc.description.abstract | UTX is a histone demethylase gene located on the X chromosome and is a frequently mutated gene in urothelial bladder cancer (UBC). UTY is a paralog of UTX located on the Y chromosome. We performed target capture sequencing on 128 genes in 40 non-metastatic UBC patients. UTX was the most frequently mutated gene (30%, 12/40). Of the genetic alterations identified, 75% were truncating mutations. UTY copy number loss was detected in 8 male patients (22.8%, 8/35). Of the 9 male patients with UTX mutations, 6 also had copy number loss (66.7%). To evaluate the functional roles of UTX and UTY in tumor progression, we designed UTX and UTY single knockout and UTX-UTY double knockout experiments using a CRISPR/Cas9 lentiviral system, and compared the proliferative capacities of two UBC cell lines in vitro. Single UTX or UTY knockout increased cell proliferation as compared to UTX-UTY wild-type cells. UTX-UTY double knockout cells exhibited greater proliferation than single knockout cells. These findings suggest both UTX and UTY function as dose-dependent suppressors of UBC development. While UTX escapes X chromosome inactivation in females, UTY may function as a male homologue of UTX, which could compensate for dosage imbalances. | * |
dc.language | English | * |
dc.publisher | IMPACT JOURNALS LLC | * |
dc.subject | urinary bladder neoplasm | * |
dc.subject | chromatin remodeling | * |
dc.subject | UTX | * |
dc.subject | UTY | * |
dc.subject | epigenesis | * |
dc.title | Target sequencing and CRISPR/Cas editing reveal simultaneous loss of UTX and UTY in urothelial bladder cancer | * |
dc.type | Article | * |
dc.relation.issue | 39 | * |
dc.relation.volume | 7 | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.startpage | 63252 | * |
dc.relation.lastpage | 63260 | * |
dc.relation.journaltitle | ONCOTARGET | * |
dc.identifier.doi | 10.18632/oncotarget.11207 | * |
dc.identifier.wosid | WOS:000387167800034 | * |
dc.identifier.scopusid | 2-s2.0-84994056268 | * |
dc.author.google | Ahn, Jinwoo | * |
dc.author.google | Kim, Kwang Hyun | * |
dc.author.google | Park, Sanghui | * |
dc.author.google | Ahn, Young-Ho | * |
dc.author.google | Kim, Ha Young | * |
dc.author.google | Yoon, Hana | * |
dc.author.google | Lee, Ji Hyun | * |
dc.author.google | Bang, Duhee | * |
dc.author.google | Lee, Dong Hyeon | * |
dc.contributor.scopusid | 이동현(24341048900;57202299352) | * |
dc.contributor.scopusid | 윤하나(8723844200) | * |
dc.contributor.scopusid | 박상희(12041890800) | * |
dc.contributor.scopusid | 안영호(7202402440) | * |
dc.contributor.scopusid | 김광현(57191527009) | * |
dc.date.modifydate | 20240222132209 | * |