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dc.contributor.author이동현*
dc.contributor.author윤하나*
dc.contributor.author박상희*
dc.contributor.author안영호*
dc.contributor.author김광현*
dc.date.accessioned2016-12-27T02:12:26Z-
dc.date.available2016-12-27T02:12:26Z-
dc.date.issued2016*
dc.identifier.issn1949-2553*
dc.identifier.otherOAK-19695*
dc.identifier.urihttps://dspace.ewha.ac.kr/handle/2015.oak/233164-
dc.description.abstractUTX is a histone demethylase gene located on the X chromosome and is a frequently mutated gene in urothelial bladder cancer (UBC). UTY is a paralog of UTX located on the Y chromosome. We performed target capture sequencing on 128 genes in 40 non-metastatic UBC patients. UTX was the most frequently mutated gene (30%, 12/40). Of the genetic alterations identified, 75% were truncating mutations. UTY copy number loss was detected in 8 male patients (22.8%, 8/35). Of the 9 male patients with UTX mutations, 6 also had copy number loss (66.7%). To evaluate the functional roles of UTX and UTY in tumor progression, we designed UTX and UTY single knockout and UTX-UTY double knockout experiments using a CRISPR/Cas9 lentiviral system, and compared the proliferative capacities of two UBC cell lines in vitro. Single UTX or UTY knockout increased cell proliferation as compared to UTX-UTY wild-type cells. UTX-UTY double knockout cells exhibited greater proliferation than single knockout cells. These findings suggest both UTX and UTY function as dose-dependent suppressors of UBC development. While UTX escapes X chromosome inactivation in females, UTY may function as a male homologue of UTX, which could compensate for dosage imbalances.*
dc.languageEnglish*
dc.publisherIMPACT JOURNALS LLC*
dc.subjecturinary bladder neoplasm*
dc.subjectchromatin remodeling*
dc.subjectUTX*
dc.subjectUTY*
dc.subjectepigenesis*
dc.titleTarget sequencing and CRISPR/Cas editing reveal simultaneous loss of UTX and UTY in urothelial bladder cancer*
dc.typeArticle*
dc.relation.issue39*
dc.relation.volume7*
dc.relation.indexSCIE*
dc.relation.indexSCOPUS*
dc.relation.startpage63252*
dc.relation.lastpage63260*
dc.relation.journaltitleONCOTARGET*
dc.identifier.doi10.18632/oncotarget.11207*
dc.identifier.wosidWOS:000387167800034*
dc.identifier.scopusid2-s2.0-84994056268*
dc.author.googleAhn, Jinwoo*
dc.author.googleKim, Kwang Hyun*
dc.author.googlePark, Sanghui*
dc.author.googleAhn, Young-Ho*
dc.author.googleKim, Ha Young*
dc.author.googleYoon, Hana*
dc.author.googleLee, Ji Hyun*
dc.author.googleBang, Duhee*
dc.author.googleLee, Dong Hyeon*
dc.contributor.scopusid이동현(24341048900;57202299352)*
dc.contributor.scopusid윤하나(8723844200)*
dc.contributor.scopusid박상희(12041890800)*
dc.contributor.scopusid안영호(7202402440)*
dc.contributor.scopusid김광현(57191527009)*
dc.date.modifydate20240222132209*


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