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Low-mass-ion discriminant equation (LOME) for ovarian cancer screening

Title
Low-mass-ion discriminant equation (LOME) for ovarian cancer screening
Authors
Lee J.H.Yoo B.C.Kim Y.H.Ahn S.-A.Yeo S.-G.Cho J.Y.Kim K.-H.Kim S.C.
Ewha Authors
김승철김윤환
SCOPUS Author ID
김승철scopus; 김윤환scopus
Issue Date
2016
Journal Title
BioData Mining
ISSN
1756-0381JCR Link
Citation
vol. 9, no. 1, pp. 1 - 14
Keywords
MALDI-TOF mass spectrometryOvarian cancerPattern recognitionScreeningSerum profiling
Publisher
BioMed Central Ltd.
Indexed
SCIE; SCOPUS WOS scopus
Abstract
Background: A low-mass-ion discriminant equation (LOME) was constructed to investigate whether systematic low-mass-ion (LMI) profiling could be applied to ovarian cancer (OVC) screening. Results: Matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) mass spectrometry was performed to obtain mass spectral data on metabolites detected as LMIs up to a mass-to-charge ratio (m/z) of 2500 for 1184 serum samples collected from healthy individuals and patients with OVC, other types of cancer, or several types of benign tumor. Principal component analysis-based discriminant analysis and two search algorithms were employed to identify discriminative low-mass ions for distinguishing OVC from non-OVC cases. OVC LOME with 13 discriminative LMIs produced excellent classification results in a validation set (sensitivity, 93.10 %; specificity, 100.0 %). Among 13 LMIs showing differential mass intensities in OVC, 3 metabolic compounds were identified and semi-quantitated. The relative amount of LPC 16:0 was somewhat decreased in OVC, but not significantly so. In contrast, D,L -glutamine and fibrinogen alpha chain fragment were significantly increased in OVC compared to the control group (p = 0.001 and 0.002, respectively). Conclusion: The present study suggested that OVC LOME might be a useful non-invasive tool with high sensitivity and specificity for OVC screening. The LOME approach could enable screening for multiple diseases, including various types of cancer, based on a single blood sample. Furthermore, the serum levels of three metabolic compounds - D,L -glutamine, LPC 16:0 and fibrinogen alpha chain fragment - might facilitate screening for OVC. © 2016 The Author(s).
DOI
10.1186/s13040-016-0111-7
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의학전문대학원 > 의학과 > Journal papers
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