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A gel-free 3D microfluidic cell culture system

Title
A gel-free 3D microfluidic cell culture system
Authors
Ong S.-M.Zhang C.Toh Y.-C.Kim S.H.Foo H.L.Tan C.H.van Noort D.Park S.Yu H.
Ewha Authors
박성수
Issue Date
2008
Journal Title
Biomaterials
ISSN
0142-9612JCR Link
Citation
vol. 29, no. 22, pp. 3237 - 3244
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
3D microfluidic cell culture systems offer a biologically relevant model to conduct micro-scale mammalian cell-based research and applications. Various natural and synthetic hydrogels have been successfully incorporated into microfluidic systems to support mammalian cells in 3D. However, embedment of cells in hydrogels introduces operational complexity, potentially hinders mass transfer, and is not suitable for establishing cell-dense, ECM-poor constructs. We present here a gel-free method for seeding and culturing mammalian cells three-dimensionally in a microfluidic channel. A combination of transient inter-cellular polymeric linker and micro-fabricated pillar arrays was used for the in situ formation and immobilization of 3D multi-cellular aggregates in a microfluidic channel. 3D cellular constructs formed this way are relieved of hydrogel embedment for cellular support. Two mammalian cell lines (A549 and C3A) and a primary mammalian cell (bone marrow mesenchymal stem cells) were cultured in the gel-free 3D microfluidic cell culture system. The cells displayed 3D cellular morphology, cellular functions and differentiation capability, affirming the versatility of the system as a 3D cell perfusion culture platform for anchorage-dependent mammalian cells. © 2008 Elsevier Ltd. All rights reserved.
DOI
10.1016/j.biomaterials.2008.04.022
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자연과학대학 > 화학·나노과학전공 > Journal papers
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