Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 이경은 | * |
dc.contributor.author | 박은미 | * |
dc.date.accessioned | 2016-12-13T02:12:27Z | - |
dc.date.available | 2016-12-13T02:12:27Z | - |
dc.date.issued | 2008 | * |
dc.identifier.issn | 0304-3940 | * |
dc.identifier.other | OAK-4916 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/233109 | - |
dc.description.abstract | Anti-inflammatory action of estrogen is involved in neuroprotection but the effects of estrogen on IL-1β and its endogenous antagonist (IL-1ra) have not been clearly defined in the ischemic brain. This study was performed to evaluate whether estrogen affects the expression of IL-1β or IL-1ra and the ratio of the two in the ischemic hippocampus. Rat organotypic hippocampal slices were treated with 17β estradiol (E2, 1 nM) for 7 days, exposed to oxygen-glucose deprivation (OGD) for 30 min, and then reperfused for 72 h. CA1 neuronal death quantified by propidium iodide (PI) staining and expressions of IL-1β and IL-1ra in slices measured by real-time PCR and Western blotting were examined. PI intensities in CA1 in slices treated with E2 were significantly reduced at 24 h and 72 h post-OGD, and IL-1β mRNA expressions were reduced at 6 h and 24 h post-OGD. In addition, IL-1ra mRNA was significantly overexpressed and the ratio of IL-1β to IL-1ra mRNA expression was reduced by E2 especially at 24 h. In terms of protein levels, E2 downregulated IL-1β but upregulated IL-1ra and thereby decreased the IL-1β/IL-1ra ratio at 24 h. These findings demonstrate that estrogen-induced protection is associated with a decrease in IL-1β and an increase in IL-1ra expression in the ischemic hippocampus during early reperfusion periods, which suggests that modulation of IL-1β/IL-1ra might be a part of anti-inflammatory effects of estrogen. © 2008 Elsevier Ireland Ltd. All rights reserved. | * |
dc.language | English | * |
dc.title | Effects of estrogen on temporal expressions of IL-1β and IL-1ra in rat organotypic hippocampal slices exposed to oxygen-glucose deprivation | * |
dc.type | Article | * |
dc.relation.issue | 2 | * |
dc.relation.volume | 438 | * |
dc.relation.index | SCI | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.startpage | 233 | * |
dc.relation.lastpage | 237 | * |
dc.relation.journaltitle | Neuroscience Letters | * |
dc.identifier.doi | 10.1016/j.neulet.2008.04.026 | * |
dc.identifier.wosid | WOS:000257276900021 | * |
dc.identifier.scopusid | 2-s2.0-43849112021 | * |
dc.author.google | Choi J.-S. | * |
dc.author.google | Kim S.-J. | * |
dc.author.google | Shin J.A. | * |
dc.author.google | Lee K.-E. | * |
dc.author.google | Park E.-M. | * |
dc.contributor.scopusid | 이경은(7409769243) | * |
dc.contributor.scopusid | 박은미(35933416400) | * |
dc.date.modifydate | 20240123095000 | * |