View : 14 Download: 0

Lactobacillus acidophilus reduces expression of enterohemorrhagic Escherichia coli O157:H7 virulence factors by inhibiting autoinducer-2-like activity

Title
Lactobacillus acidophilus reduces expression of enterohemorrhagic Escherichia coli O157:H7 virulence factors by inhibiting autoinducer-2-like activity
Authors
Kim Y.Oh S.Park S.Seo J.B.Kim S.-H.
Ewha Authors
박성수
Issue Date
2008
Journal Title
Food Control
ISSN
0956-7135JCR Link
Citation
vol. 19, no. 11, pp. 1042 - 1050
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
Recent investigations have shown that many pathogenic bacteria have quorum sensing (QS) systems to coordinate virulence factors, pointing out a new, promising target for anti-microbial drugs. In the present study, by interfering with autoinducer (AI)-2-like activity, we investigated the effects of cell extracts isolated from Lactobacillus on the virulence of enterohemorrhagic Escherichia coli (EHEC) O157:H7, including attachment, biofilm formation, and the killing of a surrogate host, the nematode Caenorhabditis elegans. In the AI-2 bioassay, profound inhibitions of AI-2-like activity were exerted on EHEC O157:H7 ATCC 43894 in the presence of the 1.0% (w/v) Lactobacillus acidophilus A4 cell extract, whereas the growth of EHEC was not changed. By adding the cell extract, inhibitory effects were exhibited on epithelial cell attachment, as well as on biofilm formation on abiotic surfaces, whereas significant reductions in EHEC O157:H7 pathogenicity were not observed in the C. elegans nematode in vivo model. In the comparative proteomic analysis, the 1.0% cell extract down-regulated expression of several virulence factors controlled by AI-2-like activity. In particular, these proteins have consistent roles in membrane associated functions and sulfur metabolism. © 2007 Elsevier Ltd. All rights reserved.
DOI
10.1016/j.foodcont.2007.10.014
Appears in Collections:
자연과학대학 > 화학·나노과학전공 > Journal papers
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE