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In vitro hepatic differentiation of human umbilical cord blood and bone marrow cells

Title
In vitro hepatic differentiation of human umbilical cord blood and bone marrow cells
Authors
Jung Y.-J.Ryu K.-H.Cho K.-A.Woo S.-Y.Seoh J.-Y.Cho S.J.Joo S.Y.Yoo K.Ho-Seoung H.
Ewha Authors
서주영유경하유권우소연조수진
SCOPUS Author ID
서주영scopus; 유경하scopus; 유권scopus; 우소연scopus; 조수진scopus
Issue Date
2008
Journal Title
Pediatric Hematology and Oncology
ISSN
0888-0018JCR Link
Citation
vol. 25, no. 6, pp. 481 - 491
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
The purpose of the present study was to investigate whether human umbilical cord blood (UCB) as well as bone marrow (BM) can generate hepatocyte lineage cells in a simple culture condition. Mononuclear cells (MNCs) separated from UCB and BM were cultured in the presence of fibroblast growth factor (FGF)-1, FGF-2, stem cell factor (SCF), and hepatocyte growth factor (HGF). The cultured cells were analyzed for morphology and for the expression of mRNAs and/or proteins of hepatocyte lineage markers. Both the UCB and BM MNCs grown in the given culture condition yielded large, round cells that were adherent to the culture dishes. RT-PCR analysis showed that mRNAs of albumin (ALB), cytokeratin (CK)-18, and alpha-fetoprotein were expressed from day 7 in both the UCB- and BM-derived cells. Immunofluorescent staining showed that the large, round cells expressed not only ALB and CK-19 but also proliferating cell nuclear antigen, implying the proliferative potential of hepatocyte lineage cells. Therefore, UCB as well as BM can give rise to hepatocyte lineage cells in the simple culture condition with HGF, SCF, FGF-1, and FGF-2. These cells may be one of the potential candidates of cell sources for the cytotherapy of hepatic disease, although it remains to be determined if the hepatocyte lineage cells are derived from plastic hematopoietic stem cells or from liver stem cells that reside in UCB or BM. Copyright © Informa Healthcare USA, Inc.
DOI
10.1080/08880010802234614
Appears in Collections:
의학전문대학원 > 의학과 > Journal papers
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