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Expression of ammonia transporters, Rhbg and Rhcg, in chronic cyclosporine nephropathy in rats

Title
Expression of ammonia transporters, Rhbg and Rhcg, in chronic cyclosporine nephropathy in rats
Authors
Lim S.W.Ahn K.O.Kim W.Y.Han D.H.Li C.Ghee J.Y.Han K.H.Kim H.-Y.Handlogten M.E.Kim J.Yang C.W.Weiner I.D.
Ewha Authors
한기환
SCOPUS Author ID
한기환scopus
Issue Date
2008
Journal Title
Nephron - Experimental Nephrology
ISSN
1660-2129JCR Link
Citation
vol. 110, no. 2, pp. e49 - e58
Indexed
SCOPUS WOS scopus
Abstract
Background/Aims: Cyclosporine (CsA)-induced renal injury causes renal tubular acidosis. The current study was performed to evaluate the influence of CsA-induced renal injury on the ammonia transporter family members, Rh B-glycoprotein (Rhbg) and Rh C-glycoprotein (Rhcg). Methods: Rats were treated daily for 1 or 4 weeks with vehicle (VH) or CsA. Induction of chronic CsA-induced nephropathy was confirmed by demonstrating impaired renal function and characteristic histopathology. Rhbg and Rhcg expression was evaluated with immunoblot, immunohistochemistry, real-time RT-PCR and electron microscopy. Results: CsA treatment for 4 weeks developed mild metabolic acidosis and decreased urinary ammonia excretion. Rhcg mRNA expression was unchanged in both the cortex and outer medulla, but Rhcg protein expression in the CsA group was significantly reduced in the cortex and outer medulla. There were no significant differences in Rhbg mRNA and protein expression between the CsA and VH group. Conclusion: Long-term treatment with CsA in rats results in decreased urinary ammonia excretion accompanied by decreased expression of Rhcg; these changes are likely to mediate the CsA-induced defect in ammonium excretion in the collecting duct. Copyright © 2008 S. Karger AG.
DOI
10.1159/000153245
Appears in Collections:
의학전문대학원 > 의학과 > Journal papers
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