View : 503 Download: 0
Hyperoside prevents oxidative damage induced by hydrogen peroxide in lung fibroblast cells via an antioxidant effect
- Title
- Hyperoside prevents oxidative damage induced by hydrogen peroxide in lung fibroblast cells via an antioxidant effect
- Authors
- Piao M.J.; Kang K.A.; Zhang R.; Ko D.O.; Wang Z.H.; You H.J.; Kim H.S.; Kim J.S.; Kang S.S.; Hyun J.W.
- Ewha Authors
- 김희선
- SCOPUS Author ID
- 김희선
- Issue Date
- 2008
- Journal Title
- Biochimica et Biophysica Acta - General Subjects
- ISSN
- 0304-4165
- Citation
- Biochimica et Biophysica Acta - General Subjects vol. 1780, no. 12, pp. 1448 - 1457
- Indexed
- SCI; SCIE; SCOPUS
- Document Type
- Article
- Abstract
- We elucidated the cytoprotective effects of hyperoside (quercetin-3-O-galactoside) against hydrogen peroxide (H2O2)-induced cell damage. We found that hyperoside scavenged the intracellular reactive oxygen species (ROS) detected by fluorescence spectrometry, flow cytometry, and confocal microscopy. In addition, we found that hyperoside scavenged the hydroxyl radicals generated by the Fenton reaction (FeSO4 + H2O2) in a cell-free system, which was detected by electron spin resonance (ESR) spectrometry. Hyperoside was found to inhibit H2O2-induced apoptosis in Chinese hamster lung fibroblast (V79-4) cells, as shown by decreased apoptotic nuclear fragmentation, decreased sub-G1 cell population, and decreased DNA fragmentation. In addition, hyperoside pretreatment inhibited the H2O2-induced activation of caspase-3 measured in terms of levels of cleaved caspase-3. Hyperoside prevented H2O2-induced lipid peroxidation as well as protein carbonyl. In addition, hyperoside prevented the H2O2-induced cellular DNA damage, which was established by comet tail, and phospho histone H2A.X expression. Furthermore, hyperoside increased the catalase and glutathione peroxidase activities. Conversely, the catalase inhibitor abolished the cytoprotective effect of hyperoside from H2O2-induced cell damage. In conclusion, hyperoside was shown to possess cytoprotective properties against oxidative stress by scavenging intracellular ROS and enhancing antioxidant enzyme activity. © 2008 Elsevier B.V. All rights reserved.
- DOI
- 10.1016/j.bbagen.2008.07.012
- Appears in Collections:
- 의과대학 > 의학과 > Journal papers
- Files in This Item:
There are no files associated with this item.
- Export
- RIS (EndNote)
- XLS (Excel)
- XML