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Induction of G1 phase arrest and apoptosis in MDA-MB-231 breast cancer cells by troglitazone, a synthetic peroxisome proliferator-activated receptor γ (PPARγ) ligand

Title
Induction of G1 phase arrest and apoptosis in MDA-MB-231 breast cancer cells by troglitazone, a synthetic peroxisome proliferator-activated receptor γ (PPARγ) ligand
Authors
Yu H.-N.Lee Y.-R.Noh E.-M.Lee K.-S.Kim J.-S.Song E.-K.Han M.-K.Lee Y.-C.Kwon K.-B.Lee S.-J.Youn H.J.Jung S.H.
Ewha Authors
이승진
SCOPUS Author ID
이승진scopus
Issue Date
2008
Journal Title
Cell Biology International
ISSN
1065-6995JCR Link
Citation
vol. 32, no. 8, pp. 906 - 912
Indexed
SCIE; SCOPUS WOS scopus
Abstract
Peroxisome proliferator-activated receptor γ (PPARγ) ligands inhibit cell proliferation and induce apoptosis in cancer cells. Here we wished to determine whether the PPARγ ligand induces apoptosis and cell cycle arrest of the MDA-MB-231 cell, an estrogen receptor α negative breast cancer cell line. The treatment of MDA-MB-231 cell with PPARγ ligands was shown to induce inhibition of cell growth in a dose-dependent manner as determined by MTT assay. Cell cycle analysis showed a G1 arrest in MDA-MB-231 cells exposed to troglitazone. An apoptotic effect by troglitazone demonstrated that apoptotic cells elevated by 2.5-fold from the control level at 10 μM, to 3.1-fold at 50 μM and to 3.5-fold at 75 μM. Moreover, troglitazone treatment, applied in a dose-dependent manner, caused a marked decrease in pRb, cyclin D1, cyclin D2, cyclin D3, Cdk2, Cdk4 and Cdk6 expression as well as a significant increase in p21 and p27 expression. These results indicate that troglitazone causes growth inhibition, G1 arrest and apoptotic death of MDA-MB-231 cells. © 2008 International Federation for Cell Biology.
DOI
10.1016/j.cellbi.2008.04.011
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약학대학 > 약학과 > Journal papers
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