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Characterization of phenotypically distinct B-cell subsets and receptor-stimulated mitogen-activated protein kinase activation in human cord blood B cells

Title
Characterization of phenotypically distinct B-cell subsets and receptor-stimulated mitogen-activated protein kinase activation in human cord blood B cells
Authors
Ha Y.J.Mun Y.-C.Seong C.-M.Lee J.R.
Ewha Authors
성주명이종란문영철
SCOPUS Author ID
성주명scopus; 이종란scopus; 문영철scopus
Issue Date
2008
Journal Title
Journal of Leukocyte Biology
ISSN
0741-5400JCR Link
Citation
vol. 84, no. 6, pp. 1557 - 1564
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
Human cord blood (CB) is a valuable source of hematopoietic stem cells, but clinical reports have indicated slow recovery of B-cell development and function after CB transplantation. To investigate the basis of these B-cell defects in reconstitution, we characterized B cells purified from CB. We compared B-cell receptor activation and B-cell subsets in CB, bone marrow (BM), and peripheral blood (PB). We found that in CB B cells activation of extracellular signal-regulated kinase (ERK) and p38 following ligation of CD40 but not of the B-cell antigen receptor (BCR) was inefficient. The patterns of expression of CD5, CD34, and CD40 in the B-cell population of CB were similar to those in PB rather than in BM. The B cells in CB contained an increased proportion of B cells expressing a high level of CD24 and a low proportion of B cells expressing CD27, pointing to the presence of circulating CD24 high immature transitional and CD27- naive B cells. CD40-mediated activation of ERK and p38 was also minimal in these B cells of CB. These findings may account for the functional defects of B cells in transplanted CB. © Society for Leukocyte Biology.
DOI
10.1189/jlb.0706457
Appears in Collections:
의과대학 > 의학과 > Journal papers
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