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Unrestrictive identification of multiple post-translational modifications from tandem mass spectrometry using an error-tolerant algorithm based on an extended sequence tag approach

Title
Unrestrictive identification of multiple post-translational modifications from tandem mass spectrometry using an error-tolerant algorithm based on an extended sequence tag approach
Authors
Na S.Jeong J.Park H.Lee K.-J.Paek E.
Ewha Authors
이공주
SCOPUS Author ID
이공주scopus
Issue Date
2008
Journal Title
Molecular and Cellular Proteomics
ISSN
1535-9476JCR Link
Citation
vol. 7, no. 12, pp. 2452 - 2463
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
Identification of post-translational modifications (PTMs) is important to understanding the biological functions of proteins. MS/MS is a useful tool to identify PTMs. Most existing search tools are restricted to take only a few types of PTMs as input. Here we describe a new algorithm, called MODi (pronounced "mod eye"), that rapidly searches for all known types of PTMs at once without limiting a multitude of modified sites in a peptide. MODi introduces the notion of a tag chain, a combination structure made from multiple sequence tags, that effectively localizes modified regions within a spectrum and overcomes de novo sequencing errors common in tag-based approaches. MODi showed its performance competence by identifying various types of PTMs in analysis of PTM- rich proteins such as glyceraldehyde-3-phosphate dehydrogenase and lens protein. We demonstrated that MODi innovatively manages the computational complexity of identifying multiple PTMs in a peptide, which may exist in a greater variety than usually expected. In addition, it is suggested tha MODi has great potential to discover novel modifications. © 2008 by The American Society for Biochemistry and Molecular Biology, Inc.
DOI
10.1074/mcp.M800101-MCP200
Appears in Collections:
약학대학 > 약학과 > Journal papers
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