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Comparison of gene-expression of MC3T3-E1 osteoblastic cell cultured on machined titanium surface and resorbable blast material titanium surface

Title
Comparison of gene-expression of MC3T3-E1 osteoblastic cell cultured on machined titanium surface and resorbable blast material titanium surface
Authors
Park S.H.Kim H.H.Lee H.S.Choi Y.S.Pae A.R.Kim M.R.
Ewha Authors
김명래
SCOPUS Author ID
김명래scopus
Issue Date
2008
Journal Title
Key Engineering Materials
ISSN
1013-9826JCR Link
Citation
vol. 361-363 II, pp. 1095 - 1098
Indexed
SCOPUS scopus
Abstract
Because of its high biocompatibility, hydroxyapatite(HA) has been considered as a good blasting material. DNA microarray is a new molecular technology that enables the analysis of gene expression in parallel on a very large number of genes, spanning a significant fraction of the human genome. It is a qualitive analysis (e.g. it can differentiate each single gene) and quantitative, since it has the sensitivity to detect a change of expression level in the investigated cells when compared to normal samples. The aim of this study is to define the cell attachment and the genetic effect of machined surface implant and RBM (resorbable blast media) surface implant on the osteoblastic cell (MC3T3-E1 osteoblastic cell line) by cDNA microarray slide containing 21575 genes. Cells were cultured on machined grade 4 titanium disks(Group 1, machined surface) and disks of RBM (Group 2) and the samples were moved to new dishes and media were added and the plated disks were cultured for 24 hours. Total RNA extraction was performed with Qiagen mini kit (Qiagen, Chatsworth, CA, USA) for microarray assay. Microarray assay after culturing the cells on the machined surface and RBM surface revealed that osteoinductive molecules appeared more prominent on the RBM surface, whereas the adhesion molecules on the biomaterial were higher on the machined surface than RBM surface.
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의학전문대학원 > 의학과 > Journal papers
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