Gene-expression of MC3T3-E1 osteoblast cells cultured on anodized titanium surface and machined titanium surface by using cDNA microarray

Abstract

One of the techniques for modifying Ti implant surface is an anodization. This method is an electrochemical one that forms rough, thick, and porous oxide films. To compare the gene expression from the cells cultured on the different implant surface, cDNA microarray have been performed. MC3T3-E1 cells were cultured on titanium disks. The control groups were cultured on the machined surfaces(MS). The experimental groups cultured on the anodized titanium surfaces(AS). Microarray assay 48 hours after culturing the cells on the machined surface and anodized surface revealed that osteoinductive molecules appeared more prominent on the anodized surface, whereas the adhesion molecules on the biomaterial were higher on the machined surface than anodized surface, which will affect the phenotype of the plated cells depending on the surface morphology.