Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 김화정 | * |
dc.contributor.author | 맹지혜 | * |
dc.date.accessioned | 2016-11-18T02:11:38Z | - |
dc.date.available | 2016-11-18T02:11:38Z | - |
dc.date.issued | 2016 | * |
dc.identifier.issn | 1422-0067 | * |
dc.identifier.other | OAK-19590 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/232697 | - |
dc.description.abstract | The translationally controlled tumor protein (TCTP), initially identified as a tumor-and growth-related protein, is also known as a histamine-releasing factor (HRF). TCTP is widely distributed in the neuronal systems, but its function is largely uncharacterized. Here, we report a novel function of TCTP in the neurotransmitter release from a neurosecretory, pheochromocytoma (PC12) cells. Treatment with recombinant TCTP (rTCTP) enhanced both basal and depolarization (50 mM KCl)-evoked [H-3]dopamine release in concentration-and time-dependent manners. Interestingly, even though rTCTP induced the increase in intracellular calcium levels ([Ca2+](i)), the rTCTP-driven effect on dopamine release was mediated by a Ca2+-independent pathway, as evidenced by the fact that Ca2+-modulating agents such as Ca2+ chelators and a voltage-gated L-type Ca2+-channel blocker did not produce any changes in rTCTP-evoked dopamine release. In a study to investigate the involvement of phospholipase A(2) (PLA(2)) in rTCTP-induced dopamine release, the inhibitor for Ca2+-independent PLA(2) (iPLA(2)) produced a significant inhibitory effect on rTCTP-induced dopamine release, whereas this release was not significantly inhibited by Ca2+-dependent cytosolic PLA(2) (cPLA(2)) and secretory PLA(2) (sPLA(2)) inhibitors. We found that rTCTP-induced dopamine release from neuronal PC12 cells was modulated by a Ca2+-independent mechanism that involved PLA(2) in the process, suggesting the regulatory role of TCTP in the neuronal functions. | * |
dc.language | English | * |
dc.publisher | MDPI AG | * |
dc.subject | translationally controlled tumor protein | * |
dc.subject | histamine releasing factor | * |
dc.subject | dopamine release | * |
dc.subject | Ca2+-independent pathways | * |
dc.subject | phospholipase A(2) | * |
dc.subject | PC12 neuronal cells | * |
dc.title | Translationally Controlled Tumor Protein Stimulates Dopamine Release from PC12 Cells via Ca2+-Independent Phospholipase A(2) Pathways | * |
dc.type | Article | * |
dc.relation.issue | 10 | * |
dc.relation.volume | 17 | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.journaltitle | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | * |
dc.identifier.doi | 10.3390/ijms17101774 | * |
dc.identifier.wosid | WOS:000387768300009 | * |
dc.identifier.scopusid | 2-s2.0-84992724793 | * |
dc.author.google | Seo, Jihui | * |
dc.author.google | Maeng, Jeehye | * |
dc.author.google | Kim, Hwa-Jung | * |
dc.contributor.scopusid | 김화정(56670336100) | * |
dc.contributor.scopusid | 맹지혜(37037712700) | * |
dc.date.modifydate | 20240118124308 | * |