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Translationally Controlled Tumor Protein Stimulates Dopamine Release from PC12 Cells via Ca2+-Independent Phospholipase A(2) Pathways
- Title
- Translationally Controlled Tumor Protein Stimulates Dopamine Release from PC12 Cells via Ca2+-Independent Phospholipase A(2) Pathways
- Authors
- Seo, Jihui; Maeng, Jeehye; Kim, Hwa-Jung
- Ewha Authors
- 김화정; 맹지혜
- SCOPUS Author ID
- 김화정; 맹지혜
- Issue Date
- 2016
- Journal Title
- INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
- ISSN
- 1422-0067
- Citation
- INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES vol. 17, no. 10
- Keywords
- translationally controlled tumor protein; histamine releasing factor; dopamine release; Ca2+-independent pathways; phospholipase A(2); PC12 neuronal cells
- Publisher
- MDPI AG
- Indexed
- SCIE; SCOPUS
- Document Type
- Article
- Abstract
- The translationally controlled tumor protein (TCTP), initially identified as a tumor-and growth-related protein, is also known as a histamine-releasing factor (HRF). TCTP is widely distributed in the neuronal systems, but its function is largely uncharacterized. Here, we report a novel function of TCTP in the neurotransmitter release from a neurosecretory, pheochromocytoma (PC12) cells. Treatment with recombinant TCTP (rTCTP) enhanced both basal and depolarization (50 mM KCl)-evoked [H-3]dopamine release in concentration-and time-dependent manners. Interestingly, even though rTCTP induced the increase in intracellular calcium levels ([Ca2+](i)), the rTCTP-driven effect on dopamine release was mediated by a Ca2+-independent pathway, as evidenced by the fact that Ca2+-modulating agents such as Ca2+ chelators and a voltage-gated L-type Ca2+-channel blocker did not produce any changes in rTCTP-evoked dopamine release. In a study to investigate the involvement of phospholipase A(2) (PLA(2)) in rTCTP-induced dopamine release, the inhibitor for Ca2+-independent PLA(2) (iPLA(2)) produced a significant inhibitory effect on rTCTP-induced dopamine release, whereas this release was not significantly inhibited by Ca2+-dependent cytosolic PLA(2) (cPLA(2)) and secretory PLA(2) (sPLA(2)) inhibitors. We found that rTCTP-induced dopamine release from neuronal PC12 cells was modulated by a Ca2+-independent mechanism that involved PLA(2) in the process, suggesting the regulatory role of TCTP in the neuronal functions.
- DOI
- 10.3390/ijms17101774
- Appears in Collections:
- 약학대학 > 약학과 > Journal papers
- Files in This Item:
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Translationally controlled tumor protein stimulates dopamine release from PC12 cells via Ca2+-independent phospholipase A2 pathways.pdf(1.78 MB)
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