View : 21 Download: 0

Dual Electrochemical Microsensor for Real-Time Simultaneous Monitoring of Nitric Oxide and Potassium Ion Changes in a Rat Brain during Spontaneous Neocortical Epileptic Seizure

Title
Dual Electrochemical Microsensor for Real-Time Simultaneous Monitoring of Nitric Oxide and Potassium Ion Changes in a Rat Brain during Spontaneous Neocortical Epileptic Seizure
Authors
Moon, JungmiHa, YejinKim, MisunSim, JeongeunLee, YoungmiSuh, Minah
Ewha Authors
이영미
SCOPUS Author ID
이영미scopus
Issue Date
2016
Journal Title
ANALYTICAL CHEMISTRY
ISSN
0003-2700JCR Link1520-6882JCR Link
Citation
vol. 88, no. 18, pp. 8942 - 8948
Publisher
AMER CHEMICAL SOC
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
In this work, we developed a dual amperometric/potentiometric microsensor for sensing nitric oxide (NO) and potassium ion (K+). The dual NO/K+ sensor was prepared :based on a dual recessed electrode possessing Pt (diameter, 50 mu m) and Ag (diameter, 76.2 mu m) microdisks. The Pt disk surface (WE1) was modified with electroplatinization and the following coating with fluorinated xerogel; and the Ag disk surface (WE2) was oxidized to AgCl on which K+ ion selective membrane was loaded-subsequent to the silanization. WE1 and WE2 of a dual microsensor were used for amperometric sensing of NO (106 +/- 28 pA mu M(-1)n = 10, at +0.85 V applied vs Ag/AgCl) and for potentiometric sensing of K+ (51.6 +/- 1.9 mV pK(-1), n = 10), respectively, with high sensitivity. In addition, the sensor showed good selectivity over common biological interferents, sufficiently fast response time and relevant stability (within 6 h in vivo experiment). The sensor had a small dimension (end plane diameter, 428 +/- 97 mu m, n = 20) and needle-like sharp geometry which allowed the sensor to be inserted in biological tissues. Taking advantage of this insertability, the sensor was applied for the simultaneous monitoring of NO and K+ changes in a living rat brain cortex at a depth of 1.19 +/- 0.039 mm and near the spontaneous epileptic seizure focus. The seizures were induced with 4-aminopyridine injection onto the rat brain cortex. NO and K+ levels were dynamically changed in clear correlation with the electrophysiological recording of seizures. This indicates that the dual NO/K+ sensor's measurements well reflect membrane potential changes of neurons and associated cellular components of neurovascular coupling. The newly developed NO/K+ dual microsensor showed the feasibility of real-time fast monitoring of dynamic changes of closely linked NO and K+ in vivo.
DOI
10.1021/acs.analchem.6b02396
Appears in Collections:
자연과학대학 > 화학·나노과학전공 > Journal papers
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE