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Hydration status affects osteopontin expression in the rat kidney

Title
Hydration status affects osteopontin expression in the rat kidney
Authors
Lee, Su-YounLee, Sae-JinPiao, Hong-LinYang, Suk-YoungWeiner, I. DavidKim, JinHan, Ki-Hwan
Ewha Authors
한기환
SCOPUS Author ID
한기환scopus
Issue Date
2016
Journal Title
JOURNAL OF VETERINARY SCIENCE
ISSN
1229-845XJCR Link1976-555XJCR Link
Citation
vol. 17, no. 3, pp. 269 - 277
Keywords
hydrationosteopontinrenal stonetubular epithelial cells
Publisher
KOREAN SOC VETERINARY SCIENCE
Indexed
SCIE; SCOPUS; KCI WOS scopus
Abstract
Osteopontin (OPN) is a secretory protein that plays an important role in urinary stone formation. Hydration status is associated with the development of urolithiasis. This study was conducted to examine the effects of dehydration and hydration on OPN expression in the rat kidney. Animals were divided into three groups, control, dehydrated, and hydrated. Kidney tissues were processed for light and electron microscope immunocytochemistry, in situ hybridization, and immunoblot analysis. Dehydration induced a significant increase in OPN protein expression, whereas increased fluid intake induced a decrease in protein expression. Under control conditions, OPN protein and mRNA expression were only detected in the descending thin limb (DTL). Dehydration induced increased expression in the DTL and the development of detectable expression in the thick ascending limb (TAL). In contrast, OPN expression levels declined to less than the controls in the DTL after hydration, while no expression of either protein or mRNA was detectable in the TAL. Immunoelectron microscopy demonstrated that hydration status altered tubular ultrastructure and intracellular OPN expression in the Golgi apparatus and secretory cytoplasmic vesicles. These data confirm that changes in oral fluid intake can regulate renal tubular epithelial cell OPN expression.
DOI
10.4142/jvs.2016.17.3.269
Appears in Collections:
의과대학 > 의학과 > Journal papers
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