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Increased sialylation and reduced fucosylation of exfoliated cervical cells are potential markers of carcinogenesis in the cervix

Title
Increased sialylation and reduced fucosylation of exfoliated cervical cells are potential markers of carcinogenesis in the cervix
Authors
Jin Y.Kim S.C.Kim H.J.Ju W.Kim Y.H.Kim H.-J.
Ewha Authors
김승철주웅김윤환
SCOPUS Author ID
김승철scopus; 주웅scopus; 김윤환scopus
Issue Date
2016
Journal Title
Clinical Chemistry and Laboratory Medicine
ISSN
1434-6621JCR Link
Citation
vol. 54, no. 11, pp. 1811 - 1819
Keywords
cervical cancerfucosylationlectinPap testsialylation
Publisher
Walter de Gruyter GmbH
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
The Pap test has been used for over 50 years for primary screening of cervical cancer. There has been no study of glycosylation changes in Pap test samples despite considerable potential of the glycosylation changes as biomarkers for detecting cancerous lesions. In this study, we developed a 96-well platform for enzyme-linked lectin assays (ELLAs) to evaluate glycosylation levels in cervical cells. A total of 117 samples of exfoliated cervical cells (ECCs) from 37 individuals with normal cytology, 20 with cervical intraepithelial neoplasia (CIN) 1, 19 with CIN 2, 26 with CIN 3 and 15 with cervical cancer were analyzed by ELLAs. The wells of 96-well plates were coated with lysates of the cervical cells, and sialylation and fucosylation levels were compared between the groups. Sialylation levels increased and fucosylation levels decreased with increasing grade of cervical dysplasia. ELLAs for sialylation [ELLA-Sambucus nigra (SNAs)] and fucosylation [ELLA-Aleuria aurantia lectin (AAL)] discriminated not only CIN 2 and worse (CIN 2+: CIN 2, CIN 3, and cancer) from normal cytology but also CIN 3 and worse (CIN 3+: CIN3 and cancer) from normal cytology. ELLA-SNAs and ELLA-AALs distinguished cancer from normal cytology with a high true-positive rate (TPR) (ELLA-SNAs: 87%; ELLA-AALs: 87%) and low false-positive rate (FPR) (ELLA-SNAs: 19%; ELLA-AALs: 11%). The sialylation and fucosylation levels of ECCs as measured by ELLAs have great potential as biomarkers for primary screening of cervical cancer. © 2016 Walter de Gruyter GmbH, Berlin/Boston.
DOI
10.1515/cclm-2015-1014
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의학전문대학원 > 의학과 > Journal papers
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