Full metadata record
DC Field | Value | Language |
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dc.contributor.author | 장남수 | - |
dc.date.accessioned | 2016-10-20T02:10:39Z | - |
dc.date.available | 2016-10-20T02:10:39Z | - |
dc.date.issued | 2009 | - |
dc.identifier.issn | 0955-2863 | - |
dc.identifier.other | OAK-5407 | - |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/232550 | - |
dc.description.abstract | We report that the maternal folate status can influence folate-mediated one-carbon metabolism and DNA methylation in the placenta. Thirty-six female Sprague-Dawley rats were divided into the following three dietary groups: folate-supplemented (FS; 8 mg/kg folic acid, n=12), homocystine- and folate-supplemented (HFS; 0.3% homocystine and 8 mg/kg folic acid, n=12) and homocystine-supplemented and folate-deficient (HFD; 0.3% homocystine and no folic acid, n=12). The animals were fed their experimental diets from 4 weeks prior to mating until Day 20 of pregnancy (n=7-9 per group). The HFS diet increased the plasma homocysteine and placental DNA methylation but did not affect plasma folate, vitamin B-12, S-adenosyl methionine (SAM) or S-adenosyl homocysteine (SAH) levels, or the SAM/SAH ratio in the liver and placenta compared with the FS diet. The HFD diet induced severely low plasma folate concentrations, with plasma homocysteine levels increasing up to 100 μmol/L, and increased hepatic SAH and decreased placental SAM levels and SAM/SAH ratio in both tissues, with a concomitant decrease in placental DNA methylation. Placental DNA methylation was significantly correlated with placental (γ=0.819), hepatic (γ=0.7) and plasma (γ=0.752) folate levels; plasma homocysteine level (γ=-0.688); hepatic SAH level (γ=-0.662) and hepatic SAM/SAH ratio (γ=0.494). These results suggest that the maternal folate status in hyperhomocysteinemic rats influences the homeostasis of folate-mediated one-carbon metabolism and the methyl pool, which would, in turn, affect placental DNA methylation by altering the methylation potential of the liver. © 2009 Elsevier Inc. All rights reserved. | - |
dc.language | English | - |
dc.title | Effect of folate deficiency on placental DNA methylation in hyperhomocysteinemic rats | - |
dc.type | Article | - |
dc.relation.issue | 3 | - |
dc.relation.volume | 20 | - |
dc.relation.index | SCI | - |
dc.relation.index | SCIE | - |
dc.relation.index | SCOPUS | - |
dc.relation.startpage | 172 | - |
dc.relation.lastpage | 176 | - |
dc.relation.journaltitle | Journal of Nutritional Biochemistry | - |
dc.identifier.doi | 10.1016/j.jnutbio.2008.01.010 | - |
dc.identifier.wosid | WOS:000263406400004 | - |
dc.identifier.scopusid | 2-s2.0-58749107929 | - |
dc.author.google | Kim J.-M. | - |
dc.author.google | Hong K. | - |
dc.author.google | Lee J.H. | - |
dc.author.google | Lee S. | - |
dc.author.google | Chang N. | - |
dc.contributor.scopusid | 장남수(7202467832;56447965800) | - |
dc.date.modifydate | 20220119163241 | - |