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Effect of folate deficiency on placental DNA methylation in hyperhomocysteinemic rats

Title
Effect of folate deficiency on placental DNA methylation in hyperhomocysteinemic rats
Authors
Kim J.-M.Hong K.Lee J.H.Lee S.Chang N.
Ewha Authors
장남수
SCOPUS Author ID
장남수scopus
Issue Date
2009
Journal Title
Journal of Nutritional Biochemistry
ISSN
0955-2863JCR Link
Citation
vol. 20, no. 3, pp. 172 - 176
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
We report that the maternal folate status can influence folate-mediated one-carbon metabolism and DNA methylation in the placenta. Thirty-six female Sprague-Dawley rats were divided into the following three dietary groups: folate-supplemented (FS; 8 mg/kg folic acid, n=12), homocystine- and folate-supplemented (HFS; 0.3% homocystine and 8 mg/kg folic acid, n=12) and homocystine-supplemented and folate-deficient (HFD; 0.3% homocystine and no folic acid, n=12). The animals were fed their experimental diets from 4 weeks prior to mating until Day 20 of pregnancy (n=7-9 per group). The HFS diet increased the plasma homocysteine and placental DNA methylation but did not affect plasma folate, vitamin B-12, S-adenosyl methionine (SAM) or S-adenosyl homocysteine (SAH) levels, or the SAM/SAH ratio in the liver and placenta compared with the FS diet. The HFD diet induced severely low plasma folate concentrations, with plasma homocysteine levels increasing up to 100 μmol/L, and increased hepatic SAH and decreased placental SAM levels and SAM/SAH ratio in both tissues, with a concomitant decrease in placental DNA methylation. Placental DNA methylation was significantly correlated with placental (γ=0.819), hepatic (γ=0.7) and plasma (γ=0.752) folate levels; plasma homocysteine level (γ=-0.688); hepatic SAH level (γ=-0.662) and hepatic SAM/SAH ratio (γ=0.494). These results suggest that the maternal folate status in hyperhomocysteinemic rats influences the homeostasis of folate-mediated one-carbon metabolism and the methyl pool, which would, in turn, affect placental DNA methylation by altering the methylation potential of the liver. © 2009 Elsevier Inc. All rights reserved.
DOI
10.1016/j.jnutbio.2008.01.010
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신산업융합대학 > 식품영양학과 > Journal papers
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