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Regulation of DUOX by the Gαq-Phospholipase Cβ-Ca2+ Pathway in Drosophila Gut Immunity

Title
Regulation of DUOX by the Gαq-Phospholipase Cβ-Ca2+ Pathway in Drosophila Gut Immunity
Authors
Ha E.-M.Lee K.-A.Park S.H.Kim S.-H.Nam H.-J.Lee H.-Y.Kang D.Lee W.-J.
Ewha Authors
이원재강동민
SCOPUS Author ID
강동민scopus
Issue Date
2009
Journal Title
Developmental Cell
ISSN
1534-5807JCR Link
Citation
vol. 16, no. 3, pp. 386 - 397
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
All metazoan guts are in constant contact with diverse food-borne microorganisms. The signaling mechanisms by which the host regulates gut-microbe interactions, however, are not yet clear. Here, we show that phospholipase C-β (PLCβ) signaling modulates dual oxidase (DUOX) activity to produce microbicidal reactive oxygen species (ROS) essential for normal host survival. Gut-microbe contact rapidly activates PLCβ through Gαq, which in turn mobilizes intracellular Ca2+ through inositol 1,4,5-trisphosphate generation for DUOX-dependent ROS production. PLCβ mutant flies had a short life span due to the uncontrolled propagation of an essential nutritional microbe, Saccharomyces cerevisiae, in the gut. Gut-specific reintroduction of the PLCβ restored efficient DUOX-dependent microbe-eliminating capacity and normal host survival. These results demonstrate that the Gαq-PLCβ-Ca2+-DUOX-ROS signaling pathway acts as a bona fide first line of defense that enables gut epithelia to dynamically control yeast during the Drosophila life cycle. © 2009 Elsevier Inc. All rights reserved.
DOI
10.1016/j.devcel.2008.12.015
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일반대학원 > 바이오융합과학과 > Journal papers
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