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SK-PC-B70M confers anti-oxidant activity and reduces Aβ levels in the brain of Tg2576 mice

Title
SK-PC-B70M confers anti-oxidant activity and reduces Aβ levels in the brain of Tg2576 mice
Authors
Seo J.-S.Kim T.-K.Leem Y.-H.Lee K.-W.Park S.-K.Baek I.-S.Kim K.-S.Im G.-J.Lee S.M.Park Y.H.Han P.-L.
Ewha Authors
한평림이강우김경심임예현
SCOPUS Author ID
한평림scopus; 임예현scopus
Issue Date
2009
Journal Title
Brain Research
ISSN
0006-8993JCR Link
Citation
vol. 1261, pp. 100 - 108
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
SK-PC-B70M is an oleanolic-glycoside saponin-enriched fraction derived from the root of Pulsatilla koreana. Recently, it was reported that hederacolchiside-E is an active ingredient of SK-PC-B70M that confers a neuroprotective effect against the cytotoxicity induced by Aβ(1-42) in SK-N-SH neuroblastoma cells. SK-PC-B70M improves scopolamine-induced impairments of spatial working memory in rats. In the present study, we investigated whether SK-PC-B70M has a beneficial effect on the Tg2576 murine model of Alzheimer's disease. ELISA analysis revealed that the levels of soluble and insoluble forms of Aβ(1-42) in Tg2576 mice fed SK-PC-B70M (2000 ppm) from 11 months to 16 months of age were reduced to, respectively, 66% and 79% of the control Tg2576 mice. Anti-Aβ antibody-stained brain sections of Tg2576 mice with SK-PC-B70M (2000 ppm) consistently showed a reduction in plaque formation in the brain. Western blot analyses showed altered expressions of various cellular factors, such as up-regulation of transthyretin, phospho-ERK, and phospho-CREB in the brain treated with SK-PC-B70M. SK-PC-B70M suppressed the neuronal toxicity induced by H2O2 in primary cortical culture. Moreover, biochemical and immunohistochemical analyses showed that the levels of malondialdehyde (MDA) and 4-hydroxy-2-nonenal (HNE), oxidized by-products of lipid peroxidation, were notably reduced in the hippocampus of Tg2576 mice treated with SK-PC-B70M compared with the Tg2576 control. These results suggest that SK-PC-B70M attenuates AD-like pathology in the brain of Tg2576 mice. © 2008 Elsevier B.V. All rights reserved.
DOI
10.1016/j.brainres.2009.01.019
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일반대학원 > 뇌·인지과학과 > Journal papers
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