Full metadata record
DC Field | Value | Language |
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dc.contributor.author | 황은숙 | * |
dc.date.accessioned | 2016-10-20T02:10:32Z | - |
dc.date.available | 2016-10-20T02:10:32Z | - |
dc.date.issued | 2009 | * |
dc.identifier.issn | 1017-7825 | * |
dc.identifier.other | OAK-5513 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/232487 | - |
dc.description.abstract | Interleukin-13 (IL-13) has been proposed as a therapeutic target for bronchial asthma as it plays crucial roles in the pathogenesis of the disease. We developed an in vitro test system measuring transcriptional downregulatory activities on IL-13 as a primary screening method to select drug candidates from natural products. The promoter region of IL-13 (-2,048 to +1) was cloned into the upstream of a luciferase gene in the plasmid pGL4.14 containing the hygromycin resistance gene as a selection marker, generating pGL4.14-IL-13. The EL-4 thymoma and RBL-2H3 mast cells transiently expressing this plasmid highly produced the luciferase activities by responding to PI (PMA and ionomycin) stimulation up to 8-fold and 13-fold compared with the control, respectively, whereas cyclosporin A, a well-known antiasthmatic agent, significantly downregulated the activities. The BF1 clone of RBL-2H3 cells constitutively expressing pGL4.14-IL-13 was established by selecting surviving cells under a constant lethal dose of hygromycin treatment. The feasibility of this system was evaluated by measuring the downregulatory activities of 354 natural products on the IL-13 promoter using the BF1 clone. An extract from Morus bombycis (named TBRC 156) significantly inhibited PI-induced luciferase activities and IL-13 mRNA expression, but not the protein expression. Fisetin (named TBRC 353) inhibited not only PI-induced luciferase activities and mRNA expression, but also the IL-13 protein secretion, whereas myricetin (named TBRC 354) could not suppress the IL-13 expression at all. Our data indicated that this in vitro test system is able to discriminate the effects on IL-13 expression, and furthermore, that it might be suitable as a simple and time-saving primary screening system to select antiasthmatic agents by measuring transcriptional activities of the IL-13 promoter. © The Korean Society for Microbiology and Biotechnology. | * |
dc.language | English | * |
dc.title | Development of an in vitro test system measuring transcriptional downregulatory activities on IL-13 | * |
dc.type | Article | * |
dc.relation.issue | 3 | * |
dc.relation.volume | 19 | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.index | KCI | * |
dc.relation.startpage | 331 | * |
dc.relation.lastpage | 337 | * |
dc.relation.journaltitle | Journal of Microbiology and Biotechnology | * |
dc.identifier.doi | 10.4014/jmb.0806.358 | * |
dc.identifier.wosid | WOS:000264757500015 | * |
dc.identifier.scopusid | 2-s2.0-64549101205 | * |
dc.author.google | Choi J.J. | * |
dc.author.google | Park B.-K. | * |
dc.author.google | Park S. | * |
dc.author.google | Yun C.-Y. | * |
dc.author.google | Kim D.H. | * |
dc.author.google | Kim J.S. | * |
dc.author.google | Hwang E.S. | * |
dc.author.google | Jin M. | * |
dc.contributor.scopusid | 황은숙(8688011100) | * |
dc.date.modifydate | 20240123102458 | * |