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DNA methylations of MC4R and HNF4 are associated with increased triglyceride levels in cord blood of preterm infants
- Title
- DNA methylations of MC4R and HNF4 are associated with increased triglyceride levels in cord blood of preterm infants
- Authors
- Kwon, Eun Jin; Lee, Hye Ah; You, Young-Ah; Park, Hyesook; Cho, Su Jin; Ha, Eun Hee; Kim, Young Ju
- Ewha Authors
- 하은희; 김영주; 박혜숙; 조수진; 유영아; 이혜아
- SCOPUS Author ID
- 하은희; 김영주; 박혜숙; 조수진; 유영아; 이혜아
- Issue Date
- 2016
- Journal Title
- MEDICINE
- ISSN
- 0025-7974
1536-5964
- Citation
- MEDICINE vol. 95, no. 35
- Keywords
- cord blood; DNA methylation; hepatocyte nuclear factor 4 alpha; melanocortin 4 receptor; metabolic indices; preterm infants; triglyceride
- Publisher
- LIPPINCOTT WILLIAMS &
WILKINS
- Indexed
- SCIE; SCOPUS
- Document Type
- Article
- Abstract
- The association of preterm birth with obesity and metabolic syndrome later in life is well established. Although the biological mechanism for this association is poorly understood, epigenetic alterations of metabolic-related genes in early life may have important roles in metabolic dysfunction. Thus, we investigated the associations of DNA methylations of melanocortin 4 receptor (MC4R) and hepatocyte nuclear factor 4 alpha (HNF4) with metabolic profiles in cord blood of term and preterm infants.We measured metabolic profiles in cord blood samples of 85 term and 85 preterm infants. DNA methylation and mRNA expression levels of MC4R and HNF4 in cord blood cells were quantified using pyrosequencing and real-time PCR. Triglyceride (TG) levels were grouped by percentile as low (<10th percentile), mid (11th-89th percentiles), and high (>90th percentile). A multiple linear regression model was used to assess the differential effects of DNA methylation on metabolic indices in cord blood between term and preterm infants.The beta-coefficients for associations between TG levels and methylation statuses of MC4R-CpG3 and HNF4-CpG2 in the P1 promoter differed significantly between term and preterm infants (P=0.04 and P=0.003, respectively). DNA methylation statuses of MC4R-CpG3 and HNF4-CpG2 in the P1 promoter were significantly lower in preterm infants in the high-TG group compared with those in the mid- and low-TG groups (P=0.01). Notably, preterm infants in the high-TG group had higher TG levels in cord blood than term infants in the high-TG group (60.49 vs 54.57mg/dL). In addition, MC4R and HNF4 expression levels were higher in preterm infants than in term infants (P<0.05).Epigenetic alterations of the newly identified genes MC4R and HNF4 in early life might contribute to metabolic profile changes, especially increased TG levels, in the cord blood of preterm infants.
- DOI
- 10.1097/MD.0000000000004590
- Appears in Collections:
- 의과대학 > 의학과 > Journal papers
- Files in This Item:
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