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Escherichia coli-derived and Staphylococcus aureus-derived extracellular vesicles induce MUC5AC expression via extracellular signal related kinase 1/2 and p38 mitogen-activated protein kinase in human airway epithelial cells

Title
Escherichia coli-derived and Staphylococcus aureus-derived extracellular vesicles induce MUC5AC expression via extracellular signal related kinase 1/2 and p38 mitogen-activated protein kinase in human airway epithelial cells
Authors
Bae, Chang HoonChoi, Yoon SeokSong, Si-YounKim, Yoon-KeunKim, Yong-Dae
Ewha Authors
김윤근
SCOPUS Author ID
김윤근scopusscopus
Issue Date
2017
Journal Title
INTERNATIONAL FORUM OF ALLERGY & RHINOLOGY
ISSN
2042-6976JCR Link2042-6984JCR Link
Citation
vol. 7, no. 1, pp. 91 - 98
Keywords
extracellular vesiclesMUC5ACERK1/2 MAPKp38 MAPKairway epithelial cell
Publisher
WILEY-BLACKWELL
Indexed
SCIE; SCOPUS WOS scopus
Abstract
Background: Escherichia coli (E. coli) and Staphylococ-cus aureus (S. aureus) release extracellular vesicles (EVs). E. coli-derived and S. aureus-derived EVs are associated with neutrophilic respiratory inflammation. In neutrophilic respiratory inflammation of human, expression of mucin is increased in airway epithelial cells and is associated with increased morbidity and mortality of the affected patients. However, no study on the effects of EVs on expression of mucin genes has been reported in airway epithelial cells. Therefore, this study was conducted in order to examine the effects and the brief signaling pathways of E. coli-derived and S. aureus-derived EVs on MUC5ACexpression in human airway epithelial cells. Methods: In mucin-producing human NCI-H292 airway ep-ithelial cells and primary cultures of normal nasal epithelial cells, the effects and signaling pathways of E. coli-derived and S. aureus-derived EVs on MUC5AC expression were examined using reverse transcription-polymerase chain reaction (RT-PCR), real-time PCR, enzyme immunoassay, and immunoblot analysis with several specific inhibitors and small interfering RNA (siRNA). Results: E. coli-derived and S. aureus-derived EVs induced MUC5ACexpression. E. coli-derived and S. aureus-derived EVs significantly activated phosphorylation of extracellu-lar signal related kinase 1/2 (ERK1/2) mitogen-activated protein kinase (MAPK) and p38 MAPK. ERK1/2 MAPK inhibitor, p38 MAPK inhibitor, ERK1/2 MAPK siRNA, and p38 MAPK siRNA significantly blocked E. coli-derived and S. aureusderived EVs induced MUC5AC messenger RNA (mRNA) expression. Conclusion: The results of this study suggest that E. coli-derived and S. aureus-derived EVs induced MUC5AC expression via ERK1/2 and p38 MAPK signaling pathways in human airway epithelial cells. (C) 2016 ARS-AAOA, LLC.
DOI
10.1002/alr.21844
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의학전문대학원 > 의학과 > Journal papers
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