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dc.contributor.author오미화-
dc.date.accessioned2017-08-28T20:06:30Z-
dc.date.available2017-08-28T20:06:30Z-
dc.date.issued2009-
dc.identifier.issn0173-0835-
dc.identifier.otherOAK-5849-
dc.identifier.urihttp://dspace.ewha.ac.kr/handle/2015.oak/232048-
dc.description.abstractRapid diagnosis of bacterial infection is important for patient management and appropriate therapy during the early phase of bacteria-induced disease. Among the existing techniques for identifying microbial, CE-SSCP combined with 16S ribosomal RNA gene-specific PCR has the benefits of excellent sensitivity, resolution, and reproducibility. However, even though CE-SSCP can separate PCR products with high-resolution, multiplex detection and quantification are complicated by primer-dimer formation and non-specific amplification. Here, we describe a novel technique for multiplex detection and quantification of pathogens by template-tagging followed by multiplex asymmetric PCR and subsequent CE-SSCP. More specifically, we reverse transcribed 16S ribosomal RNAs from seven septicemia-inducing pathogens, tagged the templates with common end sequences, and amplified them using common primers. The resulting amplicons could be successfully separated by CE-SSCP and quantified by comparison to an internal standard. This method yielded results that illustrate the potential of this system for diagnosing infectious disease. © 2009 Wiley-VCH Verlag GmbH & Co. KGaA.-
dc.languageEnglish-
dc.titleA new single-step quantitative pathogen detection system: Template-tagging followed by multiplex asymmetric PCR using common primers and CE-SSCP-
dc.typeArticle-
dc.relation.issue15-
dc.relation.volume30-
dc.relation.indexSCI-
dc.relation.indexSCIE-
dc.relation.indexSCOPUS-
dc.relation.startpage2728-
dc.relation.lastpage2736-
dc.relation.journaltitleElectrophoresis-
dc.identifier.doi10.1002/elps.200900074-
dc.identifier.wosidWOS:000269398700017-
dc.identifier.scopusid2-s2.0-70350786628-
dc.author.googleGi W.S.-
dc.author.googleYang S.C.-
dc.author.googleHee S.H.-
dc.author.googleOh M.-H.-
dc.author.googleHong G.N.-
dc.author.googleJin H.P.-
dc.author.googleGyoo Y.J.-
dc.date.modifydate20170922132039-
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엘텍공과대학 > 식품공학전공 > Journal papers
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