Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 오미화 | * |
dc.date.accessioned | 2016-08-29T11:08:35Z | - |
dc.date.available | 2016-08-29T11:08:35Z | - |
dc.date.issued | 2009 | * |
dc.identifier.issn | 0173-0835 | * |
dc.identifier.other | OAK-5849 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/232048 | - |
dc.description.abstract | Rapid diagnosis of bacterial infection is important for patient management and appropriate therapy during the early phase of bacteria-induced disease. Among the existing techniques for identifying microbial, CE-SSCP combined with 16S ribosomal RNA gene-specific PCR has the benefits of excellent sensitivity, resolution, and reproducibility. However, even though CE-SSCP can separate PCR products with high-resolution, multiplex detection and quantification are complicated by primer-dimer formation and non-specific amplification. Here, we describe a novel technique for multiplex detection and quantification of pathogens by template-tagging followed by multiplex asymmetric PCR and subsequent CE-SSCP. More specifically, we reverse transcribed 16S ribosomal RNAs from seven septicemia-inducing pathogens, tagged the templates with common end sequences, and amplified them using common primers. The resulting amplicons could be successfully separated by CE-SSCP and quantified by comparison to an internal standard. This method yielded results that illustrate the potential of this system for diagnosing infectious disease. © 2009 Wiley-VCH Verlag GmbH & Co. KGaA. | * |
dc.language | English | * |
dc.title | A new single-step quantitative pathogen detection system: Template-tagging followed by multiplex asymmetric PCR using common primers and CE-SSCP | * |
dc.type | Article | * |
dc.relation.issue | 15 | * |
dc.relation.volume | 30 | * |
dc.relation.index | SCI | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.startpage | 2728 | * |
dc.relation.lastpage | 2736 | * |
dc.relation.journaltitle | Electrophoresis | * |
dc.identifier.doi | 10.1002/elps.200900074 | * |
dc.identifier.wosid | WOS:000269398700017 | * |
dc.identifier.scopusid | 2-s2.0-70350786628 | * |
dc.author.google | Gi W.S. | * |
dc.author.google | Yang S.C. | * |
dc.author.google | Hee S.H. | * |
dc.author.google | Oh M.-H. | * |
dc.author.google | Hong G.N. | * |
dc.author.google | Jin H.P. | * |
dc.author.google | Gyoo Y.J. | * |
dc.date.modifydate | 20240322141640 | * |