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Flow cytometric analysis of Salmonella enterica serotype Typhimurium inactivated with supercritical carbon dioxide

Title
Flow cytometric analysis of Salmonella enterica serotype Typhimurium inactivated with supercritical carbon dioxide
Authors
Kim H.T.Choi H.J.Kim K.H.
Ewha Authors
최희정
SCOPUS Author ID
최희정scopus
Issue Date
2009
Journal Title
Journal of Microbiological Methods
ISSN
0167-7012JCR Link
Citation
vol. 78, no. 2, pp. 155 - 160
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
Non-thermal processes for the effective sterilization and inactivation of microorganisms are currently receiving a great deal of attention in food, pharmaceutical and other relevant industries. Supercritical carbon dioxide (SC-CO2) treatment is an alternative method of microbial inactivation that can be safely used in foods and bioactive materials at relatively low temperatures. However, to date, the inactivation of microbial cells by treatment with SC-CO2 has only been evaluated using a conventional plating method. Therefore, it is difficult to quantitatively determine the damage to cells other than counting colony forming units and also to find the possible inactivation mechanism by SC-CO2 treatment. For the first time in the area of SC-CO2 inactivation of microorganisms, we analyzed the physiological status of SC-CO2 treated Salmonella enterica serotype Typhimurium using flow cytometry and then compared the flow cytometric data to the survival rate obtained by the plating method. The results of these systemic analyses revealed that SC-CO2 caused damage to various aspects of the cells, including the efflux pump and membrane integrity. © 2009 Elsevier B.V. All rights reserved.
DOI
10.1016/j.mimet.2009.05.010
Appears in Collections:
의학전문대학원 > 의학과 > Journal papers
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