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-Gingerol suppresses interleukin-1β-induced MUC5AC gene expression in human airway epithelial cells
- -Gingerol suppresses interleukin-1β-induced MUC5AC gene expression in human airway epithelial cells
- Kim J.H.; Chang J.H.; Yoon J.-H.; Kwon S.H.; Bae J.H.; Kim K.-S.
- Ewha Authors
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- American Journal of Rhinology and Allergy
- American Journal of Rhinology and Allergy vol. 23, no. 4, pp. 385 - 391
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- Background: -Gingerol is a major active component of ginger and a natural polyphenol compound. The present study investigated whether -gingerol suppresses interleukin (IL)-1beta-induced MUC5AC gene expression in human airway epithelial cells and, if so, examined whether the suppression of MUC5AC gene expression is mediated via the mitogen-activated protein kinase (MAPK) signal transduction pathway. Methods: MUC5AC mRNA and protein were measured using reverse transcription-polymerase chain reaction (PCR), real-time PCR, and Western blot analysis in cultured NCI-H292 human airway epithelial cells. Extracellular signal-regulated kinase (ERK) and p38 MAPK protein levels were analyzed by Western blot. Results: Expression of MUC5AC mRNA increased in NCI-H292 cells upon treatment with 10 ng/mL of IL-1beta for 24 hours. When the cells were pretreated with 10 microM of -gingerol, expression of IL-1 beta -induced MUC5AC mRNA and protein was significantly suppressed. Suppression of IL-1 beta-induced MUC5AC mRNA was also observed in cells pretreated with ERK- or p38 MAPK-specific inhibitors, suggesting that -gingerol-mediated suppression of IL-1 beta -induced MUC5AC mRNA operated via the ERK- and p38 MAPK-dependent pathways. Conclusions: -Gingerol suppresses IL-1beta -induced MUC5AC gene expression in human airway epithelial cells via the ERK- and p38 MAPK-dependent pathways; therefore, -gingerol may be considered a possible anti-hypersecretory agent. Copyright © 2009, OceanSide Publications, Inc., U.S.A.
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