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Regulation of megakaryocytic differentiation of K562 cells by FosB, a member of the Fos family of AP-1 transcription factors
- Title
- Regulation of megakaryocytic differentiation of K562 cells by FosB, a member of the Fos family of AP-1 transcription factors
- Authors
- Limb J.-K.; Yoon S.; Lee K.E.; Kim B.-H.; Lee S.; Bae Y.S.; Jhon G.-J.; Kim J.
- Ewha Authors
- 전길자; 이상혁; 배윤수; 김재상; 임진경
- SCOPUS Author ID
- 전길자; 이상혁; 배윤수; 김재상; 임진경
- Issue Date
- 2009
- Journal Title
- Cellular and Molecular Life Sciences
- ISSN
- 1420-682X
- Citation
- Cellular and Molecular Life Sciences vol. 66, no. 41590, pp. 1962 - 1973
- Indexed
- SCI; SCIE; SCOPUS
- Document Type
- Article
- Abstract
- The regulation of megakaryocytic differentiation is poorly understood. Using K562 cells, which can partly recapitulate the process in response to phorbol 12-myristate 13-acetate (PMA), we performed microarray-based gene expression profiling to identify genes that play significant roles in megakaryopoiesis. Here, we describe the function of FosB, an AP-1 transcription factor. FosB is induced in PMA treated K562 cells in a sustained manner and forms an active AP-1 protein-DNA complex. Down-regulation of FosB with specific shRNAs inhibited the induction of CD41, a specific cell surface marker of megakaryocytes. We also show that activation of the PKC-MEK-ERK signaling pathway is required for induction of FosB and CD41. Finally,we cross-examined the microarray data in conjunction with gene function annotation data to identify additional target genes of FosB. We define 3 genes, INHBA, CD9, and ITGA2B as regulatory targets of FosB and show that CD9, in particular, is a direct target of FosB. © Birkhäuser Verlag, 2009.
- DOI
- 10.1007/s00018-009-8775-5
- Appears in Collections:
- 자연과학대학 > 화학·나노과학전공 > Journal papers
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