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Regulation of megakaryocytic differentiation of K562 cells by FosB, a member of the Fos family of AP-1 transcription factors

Title
Regulation of megakaryocytic differentiation of K562 cells by FosB, a member of the Fos family of AP-1 transcription factors
Authors
Limb J.-K.Yoon S.Lee K.E.Kim B.-H.Lee S.Bae Y.S.Jhon G.-J.Kim J.
Ewha Authors
전길자이상혁배윤수김재상임진경
SCOPUS Author ID
전길자scopus; 이상혁scopus; 배윤수scopus; 김재상scopus; 임진경scopus
Issue Date
2009
Journal Title
Cellular and Molecular Life Sciences
ISSN
1420-682XJCR Link
Citation
vol. 66, no. 41590, pp. 1962 - 1973
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
The regulation of megakaryocytic differentiation is poorly understood. Using K562 cells, which can partly recapitulate the process in response to phorbol 12-myristate 13-acetate (PMA), we performed microarray-based gene expression profiling to identify genes that play significant roles in megakaryopoiesis. Here, we describe the function of FosB, an AP-1 transcription factor. FosB is induced in PMA treated K562 cells in a sustained manner and forms an active AP-1 protein-DNA complex. Down-regulation of FosB with specific shRNAs inhibited the induction of CD41, a specific cell surface marker of megakaryocytes. We also show that activation of the PKC-MEK-ERK signaling pathway is required for induction of FosB and CD41. Finally,we cross-examined the microarray data in conjunction with gene function annotation data to identify additional target genes of FosB. We define 3 genes, INHBA, CD9, and ITGA2B as regulatory targets of FosB and show that CD9, in particular, is a direct target of FosB. © Birkhäuser Verlag, 2009.
DOI
10.1007/s00018-009-8775-5
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자연과학대학 > 화학·나노과학전공 > Journal papers
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