Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 최윤희 | * |
dc.date.accessioned | 2016-08-29T11:08:49Z | - |
dc.date.available | 2016-08-29T11:08:49Z | - |
dc.date.issued | 2009 | * |
dc.identifier.issn | 0891-5849 | * |
dc.identifier.other | OAK-5640 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/231929 | - |
dc.description.abstract | Several protein phosphatases are involved in neuroprotection in response to ischemic brain injury. Here, we report that reactive oxygen species (ROS)-mediated oxidative stress promotes phosphorylation of endogenous SHP-2 through lipid rafts in rat primary astrocytes. SHP-2 was transiently phosphorylated during hypoxia/reoxygenation, an effect abrogated by a ROS scavenger and an NADPH oxidase inhibitor. Additionally, exogenous treatment with hydrogen peroxide (H2O2) triggered SHP-2 phosphorylation in a time- and dose-dependent manner and led to its translocation into lipid rafts. H2O2-mediated SHP-2 phosphorylation and translocation were inhibited by filipin III and methyl-β-cyclodextrin (MCD), lipid-raft-disrupting agents. In the presence of H2O2, SHP-2 formed a complex with STAT-3 and reduced the steady-state STAT-3 phosphorylation level. Interestingly, the effect of H2O2 on SHP-2 phosphorylation was cell-type specific. Remarkably, SHP-2 phosphorylation was induced strongly by H2O2 in astrocytes, but barely detectable in microglia. Our results collectively indicate that SHP-2 is activated by ROS-mediated oxidative stress in astrocytes and functions as a component of the raft-mediated signaling pathway that acts through dephosphorylation and inactivation of other phosphotyrosine proteins, such as STAT-3. © 2009 Elsevier Inc. All rights reserved. | * |
dc.language | English | * |
dc.title | Oxidative stress induces lipid-raft-mediated activation of Src homology 2 domain-containing protein-tyrosine phosphatase 2 in astrocytes | * |
dc.type | Article | * |
dc.relation.issue | 12 | * |
dc.relation.volume | 46 | * |
dc.relation.index | SCI | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.startpage | 1694 | * |
dc.relation.lastpage | 1702 | * |
dc.relation.journaltitle | Free Radical Biology and Medicine | * |
dc.identifier.doi | 10.1016/j.freeradbiomed.2009.03.026 | * |
dc.identifier.wosid | WOS:000266358000018 | * |
dc.identifier.scopusid | 2-s2.0-67349249187 | * |
dc.author.google | Park S.J. | * |
dc.author.google | Kim H.Y. | * |
dc.author.google | Kim H. | * |
dc.author.google | Park S.M. | * |
dc.author.google | Joe E.-h. | * |
dc.author.google | Jou I. | * |
dc.author.google | Choi Y.-H. | * |
dc.contributor.scopusid | 최윤희(7404776849) | * |
dc.date.modifydate | 20240123124738 | * |