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Quantitative analysis of peripheral tissue perfusion using spatiotemporal molecular dynamics

Title
Quantitative analysis of peripheral tissue perfusion using spatiotemporal molecular dynamics
Authors
Kang Y.Choi M.Lee J.Koh G.Y.Kwon K.Choi C.
Ewha Authors
권기환
SCOPUS Author ID
권기환scopus
Issue Date
2009
Journal Title
PLoS ONE
ISSN
1932-6203JCR Link
Citation
vol. 4, no. 1
Indexed
SCIE; SCOPUS WOS scopus
Abstract
Background: Accurate measurement of peripheral tissue perfusion is challenging but necessary to diagnose peripheral vascular insufficiency. Because near infrared (NIR) radiation can penetrate relatively deep into tissue, significant attention has been given to intravital NIR fluorescence imaging. Methodology/Principal Findings: We developed a new optical imaging-based strategy for quantitative measurement of peripheral tissue perfusion by time-series analysis of local pharmacokinetics of the NIR fluorophore, indocyanine green (ICG). Time-series NIR fluorescence images were obtained after injecting ICG intravenously in a murine hindlimb ischemia model. Mathematical modeling and computational simulations were used for translating time-series ICG images into quantitative pixel perfusion rates and a perfusion map. We could successfully predict the prognosis of ischemic hindlimbs based on the perfusion profiles obtained immediately after surgery, which were dependent on the preexisting collaterals. This method also reflected increases in perfusion and improvements in prognosis of ischemic hindlimbs induced by treatment with vascular endothelial growth factor and COMP-angiopoietin-1. Conclusions/Significance: We propose that this novel NIR-imaging-based strategy is a powerful tool for biomedical studies related to the evaluation of therapeutic interventions directed at stimulating angiogenesis. © 2009 Kang et al.
DOI
10.1371/journal.pone.0004275
Appears in Collections:
의학전문대학원 > 의학과 > Journal papers
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