Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 모상준 | * |
dc.date.accessioned | 2016-08-29T11:08:18Z | - |
dc.date.available | 2016-08-29T11:08:18Z | - |
dc.date.issued | 2009 | * |
dc.identifier.issn | 0141-5492 | * |
dc.identifier.other | OAK-5375 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/231892 | - |
dc.description.abstract | Marine bacterial isolates were screened for phospholipase C (PLC) activity on PCY agar plates containing phosphatidylcholine (PC) as substrate. The strain that showed the highest activity on a PCY screening agar plate and a thin-layer chromatography was identified as a strain of Pseudoalteromonas and subsequently designated Pseudoalteromonas sp. J937. The extracellular PLC of the strain J937 was purified to a specific activity of 33 U mg-1 protein by serial ion exchange and gel filtration column chromatography. It had a molecular mass of 32 kDa estimated by SDS-PAGE. The optimal pH and temperature of the enzyme were about pH 8 and 45°C, respectively. The PLC hydrolyzed phosphatidylethanolamine as well as PC but not other glycerophospholipids. Its activity was enhanced by 150% with Ca2+ (200 mM) and by 180% with Na+ (500 mM), suggesting that the purified PLC is a marine-type enzyme. © 2008 Springer Science+Business Media B.V. | * |
dc.language | English | * |
dc.title | A novel extracellular phospholipase C purified from a marine bacterium, Pseudoalteromonas sp. J937 | * |
dc.type | Article | * |
dc.relation.issue | 1 | * |
dc.relation.volume | 31 | * |
dc.relation.index | SCI | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.startpage | 89 | * |
dc.relation.lastpage | 94 | * |
dc.relation.journaltitle | Biotechnology Letters | * |
dc.identifier.doi | 10.1007/s10529-008-9833-z | * |
dc.identifier.wosid | WOS:000262966200013 | * |
dc.identifier.scopusid | 2-s2.0-56649118967 | * |
dc.author.google | Mo S. | * |
dc.author.google | Kim J.-H. | * |
dc.author.google | Cho K.W. | * |
dc.contributor.scopusid | 모상준(23397594700) | * |
dc.date.modifydate | 20240405124155 | * |