View : 27 Download: 0

A viscosity sensitive fluorescent dye for real-time monitoring of mitochondria transport in neurons

Title
A viscosity sensitive fluorescent dye for real-time monitoring of mitochondria transport in neurons
Authors
Baek Y.Park S.J.Zhou X.Kim G.Kim H.M.Yoon J.
Ewha Authors
윤주영
SCOPUS Author ID
윤주영scopus
Issue Date
2016
Journal Title
Biosensors and Bioelectronics
ISSN
0956-5663JCR Link
Citation
vol. 86, pp. 885 - 891
Keywords
Fluorescent probeMitochondria selective probeMitochondria transport in neuronsTwo photon probeViscosity probe
Publisher
Elsevier Ltd
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
We present here a viscosity sensitive fluorescent dye, namely thiophene dihemicyanine (TDHC), that enables the specific staining of mitochondria. In comparison to the common mitochondria tracker (Mitotracker Deep Red, MTDR), this dye demonstrated its unique ability for robust staining of mitochondria with high photostability and ultrahigh signal-to-noise ratio (SNR). Moreover, TDHC also showed high sensitivity towards mitochondria membrane potential (ΔΨm) and intramitochondria viscosity change. Consequently, this dye was utilized in real-time monitoring of mitochondria transport in primary cortical neurons. Finally, the Two-Photon Microscopy (TPM) imaging ability of TDHC was also demonstrated. © 2016 Elsevier B.V.
DOI
10.1016/j.bios.2016.07.026
Appears in Collections:
자연과학대학 > 화학·나노과학전공 > Journal papers
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE