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Development of simultaneous analysis of tryptophan metabolites in serum and gastric juice – an investigation towards establishing a biomarker test for gastric cancer diagnosis

Title
Development of simultaneous analysis of tryptophan metabolites in serum and gastric juice – an investigation towards establishing a biomarker test for gastric cancer diagnosis
Authors
Choi J.M.Park W.S.Song K.Y.Lee H.J.Jung B.H.
Ewha Authors
이화정
SCOPUS Author ID
이화정scopus
Issue Date
2016
Journal Title
Biomedical Chromatography
ISSN
0269-3879JCR Link
Citation
vol. 30, no. 12, pp. 1963 - 1974
Keywords
gastric cancergastric juiceliquid chromatography–tandem mass spectrometrymetaboliteserumtryptophan
Publisher
John Wiley and Sons Ltd
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
To evaluate changes in tryptophan metabolism and discover diagnostic biomarkers for gastric cancer, a quantitative method was developed for tryptophan and its seven metabolites (indole-3-lactic acid, anthranilic acid, serotonin, nicotinic acid, kynurenic acid, kynurenine and 3-indoxyl sulfate) in both human serum and gastric juice using liquid chromatography–tandem mass spectrometry (LC–MS/MS). Serum and gastric juice were prepared with a simple protein precipitation using aqueous 0.1% formic acid and acetonitrile. As a result, it was found that the kynurenine pathway of tryptophan metabolism was activated in gastric cancer and that the metabolic ratio of kynurenine/tryptophan, which reflects the enzyme activity of indoleamine-2,3-dioxygenase, was associated with the observed metabolic changes. Finally, the investigation of tryptophan metabolites, especially kynurenic acid, in serum and gastric juice might serve as biomarkers for gastric cancer. The findings in this study provide critical information of tryptophan metabolism which can be applied to a serum-based diagnostic test for gastric cancer. Copyright © 2016 John Wiley & Sons, Ltd.
DOI
10.1002/bmc.3773
Appears in Collections:
약학대학 > 약학과 > Journal papers
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