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Heat shock protein 90 is involved in IL-17-mediated skin inflammation following thermal stimulation

Title
Heat shock protein 90 is involved in IL-17-mediated skin inflammation following thermal stimulation
Authors
Kim, Bo-KyungPark, MinhwaKim, Ji-YonLee, Kyung-HoWoo, So-Youn
Ewha Authors
우소연
SCOPUS Author ID
우소연scopus
Issue Date
2016
Journal Title
INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE
ISSN
1107-3756JCR Link1791-244XJCR Link
Citation
vol. 38, no. 2, pp. 650 - 658
Keywords
heat shock protein 90thermal effectinflammatory skin diseasekeratinocyteinterleukin-17
Publisher
SPANDIDOS PUBL LTD
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
The pathogenesis of inflammatory skin diseases involves interactions between immune cells and keratinocytes, including the T helper 17 (Th17)-mediated immune response. Several chemokines [chemokine (C-X-C motif) ligand (CXCL)1, CXCL5 and CXCL8] and antimicrobial peptides [-defensin 1 (BD1), LL-37, S100A8 and S100A9] were transcriptionally upregulated in the keratinocyte cell line HaCaT upon stimulation with interleukin (IL)-17. Balneotherapy, the treatment of disease by bathing, is an alternative therapy that has frequently been used for the treatment of inflammatory skin diseases. Immersion in pools of thermal mineral water is often considered to have chemical, thermal, mechanical and immunomodulatory benefits. We examined the effect of thermal treatment on IL-17-mediated inflammation in a model of skin disease. As Act1 is required for IL-17 signaling and is a client protein of heat shock protein 90 (HSP90), we evaluated the effect of HSP90 inhibition on IL-17-mediated cytokine and antimicrobial peptide expression in keratinocytes following heat treatment. We found that after thermal stimulation, Act1 binding to HSP90 was significantly increased in the presence of IL-17 (100 ng/ml) and 17-N-allylamino-17-demethoxygeldanamycin (17-AAG, 1 mu M). Antimicrobial peptide and chemokine expression generally increased after heat treatment; Act1 knockdown and 17-AAG reversed this effect. These observations demonstrate the possible immunomodulatory effect of heat on keratinocytes during the progression of IL-17-mediated inflammatory skin diseases.
DOI
10.3892/ijmm.2016.2627
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의학전문대학원 > 의학과 > Journal papers
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