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Elucidation of ethanol tolerance mechanisms in Saccharomyces cerevisiae by global metabolite profiling

Title
Elucidation of ethanol tolerance mechanisms in Saccharomyces cerevisiae by global metabolite profiling
Authors
Kim, SooahKim, JungyeonSong, Ju HwanJung, Young HoonChoi, Il-SupChoi, WonjaPark, Yong-CheolSeo, Jin-HoKim, Kyoung Heon
Ewha Authors
최원자
SCOPUS Author ID
최원자scopus
Issue Date
2016
Journal Title
BIOTECHNOLOGY JOURNAL
ISSN
1860-6768JCR Link1860-7314JCR Link
Citation
vol. 11, no. 9, pp. 1221 - 1229
Keywords
Ethanol toleranceMetabolite profilingMetabolomicsSaccharomyces cerevisiae
Publisher
WILEY-V C H VERLAG GMBH
Indexed
SCIE; SCOPUS WOS scopus
Abstract
Ethanol, the major fermentation product of yeast, is a stress factor in yeast. We previously constructed an ethanol-tolerant mutant yeast iETS3 by using the global transcriptional machinery engineering. However, the ethanol-tolerance mechanism has not been systematically investigated. In this study, global metabolite profiling was carried out, mainly by gas chromatography/time-of-flight mass spectrometry (GC/TOF MS), to investigate the mechanisms of ethanol tolerance in iETS3. A total of 108 intracellular metabolites were identified by GC/TOF MS and high performance liquid chromatography, and these metabolites were mostly intermediates of the central carbon metabolism. The metabolite profiles of iETS3 and BY4741, cultured with or without ethanol, were significantly different based on principal component and hierarchical clustering analyses. Our metabolomic analyses identified the compositional changes in cell membranes and the activation of glutamate metabolism and the trehalose synthetic pathway as the possible mechanisms for the ethanol tolerance. These metabolic traits can be considered possible targets for further improvement of ethanol tolerance in the mutant. For example, the KGD1 deletion mutant, with up-regulated glutamate metabolism, showed increased tolerance to ethanol. This study has demonstrated that metabolomics can be a useful tool for strain improvement and phenotypic analysis of microorganisms under stress.
DOI
10.1002/biot.201500613
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자연과학대학 > 생명과학전공 > Journal papers
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